cytochrome b5 domain containing 2 (CYB5D2) - coding DNA reference sequence

(used for variant description)

(last modified July 18, 2022)


This file was created to facilitate the description of sequence variants on transcript NM_144611.3 in the CYB5D2 gene based on a coding DNA reference sequence following the HGVS recommendations.

The sequence was taken from NC_000017.10, covering CYB5D2 transcript NM_144611.3.


Please note that introns are available by clicking on the exon numbers above the sequence.
 (upstream sequence)
                     .         .         .         .                g.5048
             atctacgtcattccgtagcctccgctgccgccatctttgttggggctg       c.-541

 .         .         .         .         .         .                g.5108
 acaacctgcaagccaagaagtacgagaagggccaggcgcggtggctcacgcctgtaatcc       c.-481

 .         .         .         .         .         .                g.5168
 cagcactttgggaggccgagacgggtggatcacctgaggtcaggagttcgagaccagcct       c.-421

 .         .         .         .         .         .                g.5228
 gaccaacgtggtgaaaccctgtctctactaaatacaaaatattagccggacgtgggggcg       c.-361

 .         .         .         .         .         .                g.5288
 cgtgcctgtaatcccagctacttgggaggctgaggcaggagaatcgcttgaacccgggag       c.-301

 .         .         .         .         .         .                g.5348
 gcggaggttgcagtgagccgagattccgccagtgcactccagcctggacaacaagagcta       c.-241

 .         .         .         .         .         .                g.5408
 aaactctgtctcagaaaaaaaaaaaaaaagtacctggaaaaagtcgcagacagcgagctt       c.-181

 .         .         .         .         .         .                g.5468
 ttcgccagtgccaggaatacagataaaacgagagagactaagggagggagcgcgagcact       c.-121

 .         .         .         .         .         .                g.5528
 agcgcgcgagagagagagcgagagcgcgcgcgccgatgacgtcacgctcggcgtctcggc       c.-61

 .         .         .         .         .         .                g.5588
 catcttagctgtagatagaggcggcaacctcggaagtgcggagcgggtgggcctatatag       c.-1

          .         .         .         .         .         .       g.5648
 ATGTTGAGGTGCGGAGGCCGTGGGCTTTTGTTGGGCCTGGCTGTAGCCGCAGCAGCGGTA       c.60
 M  L  R  C  G  G  R  G  L  L  L  G  L  A  V  A  A  A  A  V         p.20

          .         .         .         .         .         .       g.5708
 ATGGCAGCACGGCTTATGGGCTGGTGGGGTCCCCGCGCTGGCTTTCGCCTTTTCATACCG       c.120
 M  A  A  R  L  M  G  W  W  G  P  R  A  G  F  R  L  F  I  P         p.40

          .         .         .         .         .         .       g.5768
 GAGGAGCTGTCTCGCTACCGCGGCGGCCCAGGGGACCCGGGCCTGTACTTGGCGTTGCTC       c.180
 E  E  L  S  R  Y  R  G  G  P  G  D  P  G  L  Y  L  A  L  L         p.60

          .         .         .         .         .         .       g.5828
 GGCCGTGTCTACGATGTGTCCTCCGGCCGGAGGCACTACGAGCCTGGGTCCCACTATAGC       c.240
 G  R  V  Y  D  V  S  S  G  R  R  H  Y  E  P  G  S  H  Y  S         p.80

          . | 02       .         .         .         .         .    g.11773
 GGCTTCGCAG | GCCGAGACGCATCCAGAGCTTTCGTGACCGGGGACTGTTCTGAAGCAGGC    c.300
 G  F  A  G |   R  D  A  S  R  A  F  V  T  G  D  C  S  E  A  G      p.100

          .         .         .         .         .         .       g.11833
 CTCGTGGATGACGTATCCGACCTGTCAGCCGCTGAGATGCTGACACTTCACAATTGGCTT       c.360
 L  V  D  D  V  S  D  L  S  A  A  E  M  L  T  L  H  N  W  L         p.120

          .         .         .  | 03      .         .         .    g.16535
 TCATTCTATGAGAAGAATTATGTGTGTGTTG | GGAGGGTGACAGGACGGTTCTACGGAGAG    c.420
 S  F  Y  E  K  N  Y  V  C  V  G |   R  V  T  G  R  F  Y  G  E      p.140

          .         .         .         .         .         .       g.16595
 GATGGGCTGCCCACCCCGGCACTGACCCAGGTAGAAGCTGCGATCACCAGAGGCTTGGAG       c.480
 D  G  L  P  T  P  A  L  T  Q  V  E  A  A  I  T  R  G  L  E         p.160

          .         .         .         .         .         .       g.16655
 GCCAACAAACTACAGCTGCAAGAGAAGCAGACATTCCCGCCGTGCAACGCGGAGTGGAGC       c.540
 A  N  K  L  Q  L  Q  E  K  Q  T  F  P  P  C  N  A  E  W  S         p.180

          .         .         .         | 04         .         .    g.18720
 TCAGCCAGGGGCAGCCGGCTCTGGTGCTCCCAGAAGAG | TGGAGGTGTGAGCAGAGACTGG    c.600
 S  A  R  G  S  R  L  W  C  S  Q  K  S  |  G  G  V  S  R  D  W      p.200

          .         .         .         .         .         .       g.18780
 ATTGGCGTCCCCAGGAAGCTGTATAAGCCAGGTGCTAAGGAGCCCCGCTGCGTGTGTGTG       c.660
 I  G  V  P  R  K  L  Y  K  P  G  A  K  E  P  R  C  V  C  V         p.220

          .         .         .         .         .         .       g.18840
 AGAACCACCGGCCCCCCTAGTGGCCAGATGCCGGACAACCCTCCACACAGAAATCGTGGG       c.720
 R  T  T  G  P  P  S  G  Q  M  P  D  N  P  P  H  R  N  R  G         p.240

          .         .         .         .         .         .       g.18900
 GACCTGGACCACCCAAACTTGGCAGAGTACACAGGCTGCCCACCGCTAGCCATCACATGC       c.780
 D  L  D  H  P  N  L  A  E  Y  T  G  C  P  P  L  A  I  T  C         p.260

          .                                                         g.18915
 TCCTTTCCACTCTAA                                                    c.795
 S  F  P  L  X                                                      p.264

          .         .         .         .         .         .       g.18975
 gccgtagcctcttctgttaataacacacagagagctctgccaagcacctgagtaggccct       c.*60

          .         .         .         .         .         .       g.19035
 tgacacttgtgtgccctgggatgcctcctggcgcgaatcaggagggtctggaaggactct       c.*120

          .         .         .         .         .         .       g.19095
 ggctatattctgcaaatgtggctcatgccccttaccgtggctcggcgttgtggtgcctga       c.*180

          .         .         .         .         .         .       g.19155
 gggacagccggccacctgcccagtactggtcagcttttcaacactattccctttgaccta       c.*240

          .         .         .         .         .         .       g.19215
 ctggccatcttcctcacagccctcagatatcaacgggcacaaataagaccaactcaattt       c.*300

          .         .         .         .         .         .       g.19275
 ccacttgaatttacaaccaaaagcctgctgagttgattacagctgggccaatacagtacg       c.*360

          .         .         .         .         .         .       g.19335
 aggcaataacaaattagtgtgggttgattctggaattggaaaagcttttgcttgtatgga       c.*420

          .         .         .         .         .         .       g.19395
 tacagcaaatccagatgtctctgaacaaagcaacaatttaaagcaacgacattttctgtc       c.*480

          .         .         .         .         .         .       g.19455
 ctttaagcacttaaaatcaggtgtggtgtgttttcaaaggcagaagtctgcattttgagc       c.*540

          .         .         .         .         .         .       g.19515
 aaaaggtggcttcccagctctaacaaggtaactggttagcatgacattaaagcttgggca       c.*600

          .                                                         g.19530
 aggcttcaaacttaa                                                    c.*615

 (downstream sequence)
Legend:
Nucleotide numbering (following the rules of the HGVS for a 'Coding DNA Reference Sequence') is indicated at the right of the sequence, counting the A of the ATG translation initiating Methionine as 1. Every 10th nucleotide is indicated by a "." above the sequence. The Cytochrome b5 domain containing 2 protein sequence is shown below the coding DNA sequence, with numbering indicated at the right starting with 1 for the translation initiating Methionine. Every 10th amino acid is shown in bold. The position of introns is indicated by a vertical line, splitting the two exons. The start of the first exon (transcription initiation site) is indicated by a '\', the end of the last exon (poly-A addition site) by a '/'. The exon number is indicated above the first nucleotide(s) of the exon. To aid the description of frame shift variants, all stop codons in the +1 frame are shown in bold while all stop codons in the +2 frame are underlined.

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