excision repair cross-complementing rodent repair deficiency, complementation group 8 (ERCC8) - coding DNA reference sequence

(used for variant description)

(last modified November 25, 2015)


This file was created to facilitate the description of sequence variants on transcript NM_000082.3 in the ERCC8 gene based on a coding DNA reference sequence following the HGVS recommendations.

The sequence was taken from NG_009289.1, covering ERCC8 transcript NM_000082.3.


Please note that introns are available by clicking on the exon numbers above the sequence.
 (upstream sequence)
                                                   .                g.5010
                                                   gctgttccag       c.-61

 .         .         .         .         .         .                g.5070
 tcccgcccctgagctctgtcatggcgacgtccagtgctccagccggtgtgaggacacgat       c.-1

          .         .         .         .         .         .       g.5130
 ATGCTGGGGTTTTTGTCCGCACGCCAAACGGGTTTGGAGGACCCTCTTCGCCTTCGGAGA       c.60
 M  L  G  F  L  S  A  R  Q  T  G  L  E  D  P  L  R  L  R  R         p.20

          .        | 02.         .         .         .         .    g.21162
 GCAGAGTCAACACGGAG | AGTTTTGGGACTGGAATTAAATAAAGACAGAGATGTTGAAAGA    c.120
 A  E  S  T  R  R  |  V  L  G  L  E  L  N  K  D  R  D  V  E  R      p.40

          .         .         .         .         .    | 03    .    g.27930
 ATCCACGGCGGTGGAATTAACACCCTTGACATTGAACCTGTTGAAGGGAGATA | CATGTTA    c.180
 I  H  G  G  G  I  N  T  L  D  I  E  P  V  E  G  R  Y  |  M  L      p.60

          .         .         .         .         .         .       g.27990
 TCAGGTGGTTCAGATGGTGTGATTGTACTTTATGACCTTGAGAACTCCAGCAGACAATCT       c.240
 S  G  G  S  D  G  V  I  V  L  Y  D  L  E  N  S  S  R  Q  S         p.80

          .         .         .      | 04  .         .         .    g.31715
 TATTACACATGTAAAGCAGTGTGTTCCATTGGCAG | AGATCATCCTGATGTTCACAGATAC    c.300
 Y  Y  T  C  K  A  V  C  S  I  G  R  |  D  H  P  D  V  H  R  Y      p.100

          .         .         .         .         .         .       g.31775
 AGTGTGGAGACTGTACAGTGGTATCCTCATGACACTGGCATGTTCACATCAAGCTCATTT       c.360
 S  V  E  T  V  Q  W  Y  P  H  D  T  G  M  F  T  S  S  S  F         p.120

          .         .         .          | 05        .         .    g.45226
 GATAAAACTCTGAAAGTATGGGATACAAATACATTACAA | ACTGCAGATGTATTTAATTTT    c.420
 D  K  T  L  K  V  W  D  T  N  T  L  Q   | T  A  D  V  F  N  F      p.140

          .         .         .         .         .         .       g.45286
 GAGGAAACAGTTTATAGTCATCATATGTCTCCAGTCTCCACCAAGCACTGTTTGGTAGCA       c.480
 E  E  T  V  Y  S  H  H  M  S  P  V  S  T  K  H  C  L  V  A         p.160

   | 06      .         .         .         .         .         .    g.46421
 G | TTGGTACTAGAGGACCCAAAGTACAACTTTGTGACTTGAAGTCTGGATCCTGTTCTCAC    c.540
 V |   G  T  R  G  P  K  V  Q  L  C  D  L  K  S  G  S  C  S  H      p.180

          . | 07       .         .         .         .         .    g.47620
 ATTCTACAGG | GTCACAGACAAGAAATATTAGCAGTTTCCTGGTCTCCACGTTATGACTAT    c.600
 I  L  Q  G |   H  R  Q  E  I  L  A  V  S  W  S  P  R  Y  D  Y      p.200

          .        | 08.         .         .         .         .    g.50394
 ATCTTGGCAACAGCAAG | TGCTGACAGTAGAGTAAAATTATGGGATGTGAGAAGAGCATCA    c.660
 I  L  A  T  A  S  |  A  D  S  R  V  K  L  W  D  V  R  R  A  S      p.220

          .         .         .         .         .         | 09    g.51680
 GGATGTTTGATTACTCTTGATCAACATAATGGGAAAAAGTCACAAGCTGTTGAATCAG | CA    c.720
 G  C  L  I  T  L  D  Q  H  N  G  K  K  S  Q  A  V  E  S  A |       p.240

          .         .         .         .         .         .       g.51740
 AACACTGCTCATAATGGGAAAGTTAATGGCTTATGTTTTACAAGTGATGGACTTCACCTC       c.780
 N  T  A  H  N  G  K  V  N  G  L  C  F  T  S  D  G  L  H  L         p.260

          .         .         .         .         .         .       g.51800
 CTCACTGTTGGTACAGATAATCGAATGAGGCTCTGGAATAGTTCCAATGGAGAAAACACA       c.840
 L  T  V  G  T  D  N  R  M  R  L  W  N  S  S  N  G  E  N  T         p.280

     | 10    .         .         .         .         .         .    g.59049
 CTT | GTGAACTATGGAAAAGTTTGTAATAACAGTAAAAAAGGATTGAAATTCACTGTCTCC    c.900
 L   | V  N  Y  G  K  V  C  N  N  S  K  K  G  L  K  F  T  V  S      p.300

          .         .         .         .         .         .       g.59109
 TGTGGCTGCAGTTCAGAATTTGTTTTTGTACCATATGGTAGCACCATTGCTGTTTATACA       c.960
 C  G  C  S  S  E  F  V  F  V  P  Y  G  S  T  I  A  V  Y  T         p.320

          .         .         .         .         .         .       g.59169
 GTTTACTCAGGAGAACAGATAACTATGCTTAAGGGACATTATAAAACTGTTGACTGCTGT       c.1020
 V  Y  S  G  E  Q  I  T  M  L  K  G  H  Y  K  T  V  D  C  C         p.340

          .         .  | 11      .         .         .         .    g.62597
 GTATTTCAGTCAAATTTCCAG | GAACTTTATAGTGGTAGCAGAGACTGCAACATTCTGGCT    c.1080
 V  F  Q  S  N  F  Q   | E  L  Y  S  G  S  R  D  C  N  I  L  A      p.360

          .         .         .         .   | 12     .         .    g.75413
 TGGGTTCCATCCTTATATGAACCAGTTCCTGATGATGATGAG | ACTACAACAAAATCACAA    c.1140
 W  V  P  S  L  Y  E  P  V  P  D  D  D  E   | T  T  T  K  S  Q      p.380

          .         .         .         .         .                 g.75464
 TTAAATCCGGCCTTTGAAGATGCCTGGAGCAGCAGTGATGAAGAAGGATGA                c.1191
 L  N  P  A  F  E  D  A  W  S  S  S  D  E  E  G  X                  p.396

          .         .         .         .         .         .       g.75524
 atatcatctttagtacctttttgtctctgctgaaactttttaaatgagactgtgtttttt       c.*60

          .         .         .         .         .         .       g.75584
 tcaactgtatggtctattcctgacagctaaattagccctaaatgtgggtaatatttttcc       c.*120

          .         .         .         .         .         .       g.75644
 tcatgttttaaaatgaggttaatatttgcataaaatcctaaaacagacttctgtatagtt       c.*180

          .         .         .         .         .         .       g.75704
 tatttagtcaaaatgtgttccttgatcccagatgttgtggcctgggaaagccctcattgc       c.*240

          .         .         .         .         .         .       g.75764
 tacagtacaagtaacacaagtcgttgtacctcagttgtgaccttcagcagattttatgaa       c.*300

          .         .         .         .         .         .       g.75824
 ctataagatgcagtctcagaggatcagcaagtggaggccatcagtattgactttctctta       c.*360

          .         .         .         .         .         .       g.75884
 cttgctgtactatcagcctgctcgtttccacctttaagaatgattttgccaagaatgatt       c.*420

          .         .         .         .         .         .       g.75944
 atatcaaaaatagtagttgaaatggtaacatcaaaattattttattctttcttcttcatg       c.*480

          .         .         .         .         .         .       g.76004
 tattcacatttttcagtggtttcatttaattaaccatgctttatgttaaacattttgggg       c.*540

          .         .         .         .         .         .       g.76064
 ctcaatgtctcctactatccaaaatgtgcatcacaggaggcttttaactttgtgaaaatc       c.*600

          .         .         .         .         .         .       g.76124
 ccatgtttgctttattttattttaatgtcagaaggcagtttgcgctaatgcttgaactct       c.*660

          .         .         .         .         .         .       g.76184
 ttttctgtgaaactcattaaggtatgaccaaatcctgcctcattaattcaagcagaaaat       c.*720

          .         .         .         .         .         .       g.76244
 atcctggcagggaatctggcttaaacatgaaatgctgtaataaaatttctatgttattgt       c.*780

                                                                    g.76247
 ctc                                                                c.*783

 (downstream sequence)
Legend:
Nucleotide numbering (following the rules of the HGVS for a 'Coding DNA Reference Sequence') is indicated at the right of the sequence, counting the A of the ATG translation initiating Methionine as 1. Every 10th nucleotide is indicated by a "." above the sequence. The Excision repair cross-complementing rodent repair deficiency, complementation group 8 protein sequence is shown below the coding DNA sequence, with numbering indicated at the right starting with 1 for the translation initiating Methionine. Every 10th amino acid is shown in bold. The position of introns is indicated by a vertical line, splitting the two exons. The start of the first exon (transcription initiation site) is indicated by a '\', the end of the last exon (poly-A addition site) by a '/'. The exon number is indicated above the first nucleotide(s) of the exon. To aid the description of frame shift variants, all stop codons in the +1 frame are shown in bold while all stop codons in the +2 frame are underlined.

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