keratocan (KERA) - coding DNA reference sequence

(used for variant description)

(last modified August 30, 2012)


This file was created to facilitate the description of sequence variants on transcript NM_007035.3 in the KERA gene based on a coding DNA reference sequence following the HGVS recommendations.

The sequence was taken from NC_000012.11, covering KERA transcript NM_007035.3.


Please note that introns are available by clicking on the exon numbers above the sequence.
 (upstream sequence)
                                                   .                g.5019
                                          caatttttaagaaatgatc       c.-601

 .         .         .         .         .         .                g.5079
 atctagggaaaatgttttaaaatcttttaaaatatctttttgttatactttaaaactgcc       c.-541

 .         .         .         .         .         .                g.5139
 ttcagctgcctattggtaaataaggtctggatatcagtacatgacaacatatactaatga       c.-481

 .         .         .         .         .         .                g.5199
 attgaactgtgaattaggaatgaaaattgcctagaaatttttttatgagttattaggatt       c.-421

 .         .         .         .         .         .                g.5259
 aaagtaagaatttcctttgctgggctgtgtaggcactaagattccgtggttgttggaggg       c.-361

 .         .         .         .         .         .                g.5319
 gttaaatttggatctactagtgcatatattttaccgaggattcataaaaacacgacagtg       c.-301

 .         .         .         .         .         .                g.5379
 gagcctgggtcaatccattaccatgtacaagcagtgaccaatagaaaaggaagaagaaga       c.-241

 .         .         .         .         .         .                g.5439
 tccaagaaggctgaggtaagcagtcttctgaagccctgtcaaagtggaaacaatagatca       c.-181

 .         .         .         .         .         .                g.5499
 aaggtgttcatggtgactgggacgagtaggtttcactgtttctcatagacgacttgacag       c.-121

 .         .         .         .         .         .                g.5559
 cttaaagtaaaaacaaattattttcgtcaaagtttttttttttctcttaactgattttta       c.-61

 .         .         .         .         .         .  | 02          g.7073
 gcaaacctcagactgagacacaggactcaacggtgtattcctggaaggcaag | gtgctata    c.-1

          .         .         .         .         .         .       g.7133
 ATGGCAGGCACAATCTGTTTCATCATGTGGGTGTTATTCATAACAGACACTGTGTGGTCT       c.60
 M  A  G  T  I  C  F  I  M  W  V  L  F  I  T  D  T  V  W  S         p.20

          .         .         .         .         .         .       g.7193
 AGAAGTGTGAGGCAGGTCTATGAAGTACATGATTCAGATGATTGGACTATTCATGACTTC       c.120
 R  S  V  R  Q  V  Y  E  V  H  D  S  D  D  W  T  I  H  D  F         p.40

          .         .         .         .         .         .       g.7253
 GAGTGTCCCATGGAATGTTTCTGCCCACCCAGTTTTCCTACTGCTTTATATTGTGAAAAT       c.180
 E  C  P  M  E  C  F  C  P  P  S  F  P  T  A  L  Y  C  E  N         p.60

          .         .         .         .         .         .       g.7313
 AGAGGTCTCAAAGAAATTCCTGCTATTCCTTCAAGAATTTGGTATCTTTATCTTCAAAAC       c.240
 R  G  L  K  E  I  P  A  I  P  S  R  I  W  Y  L  Y  L  Q  N         p.80

          .         .         .         .         .         .       g.7373
 AACCTGATAGAAACCATTCCTGAAAAGCCATTTGAGAATGCCACCCAGCTAAGATGGATA       c.300
 N  L  I  E  T  I  P  E  K  P  F  E  N  A  T  Q  L  R  W  I         p.100

          .         .         .         .         .         .       g.7433
 AATCTAAACAAGAACAAAATAACCAACTACGGAATTGAAAAAGGAGCCCTAAGCCAGCTG       c.360
 N  L  N  K  N  K  I  T  N  Y  G  I  E  K  G  A  L  S  Q  L         p.120

          .         .         .         .         .         .       g.7493
 AAGAAGTTGCTCTTCTTATTTCTGGAAGATAATGAGCTAGAGGAGGTACCTTCTCCATTG       c.420
 K  K  L  L  F  L  F  L  E  D  N  E  L  E  E  V  P  S  P  L         p.140

          .         .         .         .         .         .       g.7553
 CCAAGAAGTTTAGAACAATTACAATTAGCTAGAAATAAGGTGTCCAGAATTCCTCAAGGG       c.480
 P  R  S  L  E  Q  L  Q  L  A  R  N  K  V  S  R  I  P  Q  G         p.160

          .         .         .         .         .         .       g.7613
 ACCTTTAGCAATCTGGAGAACCTGACCCTTCTTGACCTACAGAACAACAAATTAGTGGAC       c.540
 T  F  S  N  L  E  N  L  T  L  L  D  L  Q  N  N  K  L  V  D         p.180

          .         .         .         .         .         .       g.7673
 AATGCCTTTCAAAGAGACACTTTTAAAGGACTCAAGAATCTCATGCAGCTAAACATGGCC       c.600
 N  A  F  Q  R  D  T  F  K  G  L  K  N  L  M  Q  L  N  M  A         p.200

          .         .         .         .         .         .       g.7733
 AAGAATGCCCTGAGGAATATGCCTCCAAGATTACCAGCCAATACAATGCAGTTGTTTTTA       c.660
 K  N  A  L  R  N  M  P  P  R  L  P  A  N  T  M  Q  L  F  L         p.220

          .         .         .         .         .         .       g.7793
 GACAACAATTCCATTGAAGGAATACCAGAAAATTATTTTAATGTGATTCCTAAAGTGGCC       c.720
 D  N  N  S  I  E  G  I  P  E  N  Y  F  N  V  I  P  K  V  A         p.240

          .         .         .         .         .         .       g.7853
 TTTTTGAGACTAAATCACAACAAACTGTCAGATGAGGGTCTCCCATCAAGAGGATTTGAT       c.780
 F  L  R  L  N  H  N  K  L  S  D  E  G  L  P  S  R  G  F  D         p.260

          .         .         .         .         .         .       g.7913
 GTATCATCAATTCTAGATCTTCAACTGTCGCACAATCAACTCACAAAGGTTCCCCGAATC       c.840
 V  S  S  I  L  D  L  Q  L  S  H  N  Q  L  T  K  V  P  R  I         p.280

          .         .         .         .       | 03 .         .    g.11850
 AGTGCTCATCTGCAGCACCTTCACCTTGATCATAACAAAATTAAAA | GTGTGAATGTCTCT    c.900
 S  A  H  L  Q  H  L  H  L  D  H  N  K  I  K  S |   V  N  V  S      p.300

          .         .         .         .         .         .       g.11910
 GTAATATGTCCCAGCCCATCCATGCTGCCTGCAGAACGAGATTCCTTCAGTTATGGACCT       c.960
 V  I  C  P  S  P  S  M  L  P  A  E  R  D  S  F  S  Y  G  P         p.320

          .         .         .         .         .         .       g.11970
 CATCTTCGCTACCTCCGTCTGGATGGAAATGAAATCAAACCACCAATTCCAATGGCTTTA       c.1020
 H  L  R  Y  L  R  L  D  G  N  E  I  K  P  P  I  P  M  A  L         p.340

          .         .         .                                     g.12009
 ATGACCTGCTTCAGACTTCTGCAGGCTGTCATTATTTAA                            c.1059
 M  T  C  F  R  L  L  Q  A  V  I  I  X                              p.352

          .         .         .         .         .         .       g.12069
 acacattctcaccaaatctaaaattagtttaatgagctgtttctgacatgaaatgtggtt       c.*60

          .         .         .         .         .         .       g.12129
 accattaataggtttaggacacaagtcatattccccattgctctcggccaccattttcat       c.*120

          .         .         .         .         .         .       g.12189
 ttgtgcagtgtattttttctattcaaagatgcttttgccagttacatgcatcacagcctg       c.*180

          .         .         .         .         .         .       g.12249
 cattaatttgcttttcttttaattaataaaacagacacagagttaagatagtttatcaac       c.*240

          .         .         .         .         .         .       g.12309
 tcaaagatagttttattttggtctcttccatagcttattaacactaaagaaaacaattac       c.*300

          .         .         .         .         .         .       g.12369
 attcttatacaataaaaaggacacatttgtgtatgtttaaaattacttatgcagataccg       c.*360

          .         .         .         .         .         .       g.12429
 taatttacagtataaatgtaataatcaaacaggaggataacgacctgaagaaaatgagaa       c.*420

          .         .         .         .         .         .       g.12489
 aataaaatatttcttaattgtaatcataataaaacaattgcagattgctaagtagctgtt       c.*480

          .         .         .         .         .         .       g.12549
 ggtggtacaagtttgatatttagcttgagaatgggtatggaatgaatcaaatacttcatc       c.*540

          .         .         .         .         .         .       g.12609
 actaaagaattcttgttatttaatatcaaataatgaaacagcagtcacttctagttcatt       c.*600

          .         .         .         .         .         .       g.12669
 accatttcttgtagctgttgtttttagatatagtccactgcattcttataagttctaaat       c.*660

          .         .         .         .         .         .       g.12729
 tttatgtttattataatgcatttcttgtggaatcttagtgttttaacatttgaaatccac       c.*720

          .         .         .         .         .         .       g.12789
 ttaaactgcaaattgttctttatatttgattgtgttttttggattttctaaataaaaata       c.*780

          .         .         .         .         .         .       g.12849
 ttgaatgctttctaacaaaacagaatatttcaaagaaattagcaagcttcattaaagaac       c.*840

          .                                                         g.12864
 atgttttaataaaaa                                                    c.*855

 (downstream sequence)
Legend:
Nucleotide numbering (following the rules of the HGVS for a 'Coding DNA Reference Sequence') is indicated at the right of the sequence, counting the A of the ATG translation initiating Methionine as 1. Every 10th nucleotide is indicated by a "." above the sequence. The Keratocan protein sequence is shown below the coding DNA sequence, with numbering indicated at the right starting with 1 for the translation initiating Methionine. Every 10th amino acid is shown in bold. The position of introns is indicated by a vertical line, splitting the two exons. The start of the first exon (transcription initiation site) is indicated by a '\', the end of the last exon (poly-A addition site) by a '/'. The exon number is indicated above the first nucleotide(s) of the exon. To aid the description of frame shift variants, all stop codons in the +1 frame are shown in bold while all stop codons in the +2 frame are underlined.

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