neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS) - coding DNA reference sequence

(used for variant description)

(last modified March 27, 2015)

This file was created to facilitate the description of sequence variants on transcript NM_002524.4 in the NRAS gene based on a coding DNA reference sequence following the HGVS recommendations.

The sequence was taken from NC_000001.10, covering NRAS transcript NM_002524.4.

Please note that introns are available by clicking on the exon numbers above the sequence.

 (upstream sequence)
                                                   .                g.5014
                                               gaaacgtcccgtgt       c.-241

 .         .         .         .         .         .                g.5074
 gggaggggcgggtctgggtgcggcctgccgcatgactcgtggttcggaggcccacgtggc       c.-181

 .         .         .         .         .         .                g.5134
 cggggcggggactcaggcgcctggggcgccgactgattacgtagcgggcggggccggaag       c.-121

 .         .         .         .         .         .                g.5194
 tgccgctccttggtgggggctgttcatggcggttccggggtctccaacatttttcccggc       c.-61

 .         .         .         .         .   | 02     .             g.5734
 tgtggtcctaaatctgtccaaagcagaggcagtggagcttgag | gttcttgctggtgtgaa    c.-1

          .         .         .         .         .         .       g.5794
 ATGACTGAGTACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA       c.60
 M  T  E  Y  K  L  V  V  V  G  A  G  G  V  G  K  S  A  L  T         p.20

          .         .         .         .         .  | 03      .    g.7925
 ATCCAGCTAATCCAGAACCACTTTGTAGATGAATATGATCCCACCATAGAG | GATTCTTAC    c.120
 I  Q  L  I  Q  N  H  F  V  D  E  Y  D  P  T  I  E   | D  S  Y      p.40

          .         .         .         .         .         .       g.7985
 AGAAAACAAGTGGTTATAGATGGTGAAACCTGTTTGTTGGACATACTGGATACAGCTGGA       c.180
 R  K  Q  V  V  I  D  G  E  T  C  L  L  D  I  L  D  T  A  G         p.60

          .         .         .         .         .         .       g.8045
 CAAGAAGAGTACAGTGCCATGAGAGACCAATACATGAGGACAGGCGAAGGCTTCCTCTGT       c.240
 Q  E  E  Y  S  A  M  R  D  Q  Y  M  R  T  G  E  G  F  L  C         p.80

          .         .         .         .         . | 04       .    g.12176
 GTATTTGCCATCAATAATAGCAAGTCATTTGCGGATATTAACCTCTACAG | GGAGCAGATT    c.300
 V  F  A  I  N  N  S  K  S  F  A  D  I  N  L  Y  R  |  E  Q  I      p.100

          .         .         .         .         .         .       g.12236
 AAGCGAGTAAAAGACTCGGATGATGTACCTATGGTGCTAGTGGGAAACAAGTGTGATTTG       c.360
 K  R  V  K  D  S  D  D  V  P  M  V  L  V  G  N  K  C  D  L         p.120

          .         .         .         .         .         .       g.12296
 CCAACAAGGACAGTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCA       c.420
 P  T  R  T  V  D  T  K  Q  A  H  E  L  A  K  S  Y  G  I  P         p.140

          .         .         . | 05       .         .         .    g.13270
 TTCATTGAAACCTCAGCCAAGACCAGACAG | GGTGTTGAAGATGCTTTTTACACACTGGTA    c.480
 F  I  E  T  S  A  K  T  R  Q   | G  V  E  D  A  F  Y  T  L  V      p.160

          .         .         .         .         .         .       g.13330
 AGAGAAATACGCCAGTACCGAATGAAAAAACTCAACAGCAGTGATGATGGGACTCAGGGT       c.540
 R  E  I  R  Q  Y  R  M  K  K  L  N  S  S  D  D  G  T  Q  G         p.180

          .         .         .                                 g.13360
 TGTATGGGATTGCCATGTGTGGTGATGTAA |                                  c.571
 C  M  G  L  P  C  V  V  M  X                                    p.189

      | 06   .         .         .         .    | 07    .         . g.13861
 caag | atacttttaaagttttgtcagaaaagagccactttcaag | ctgcactgacaccctgg c.*60

          .         .         .         .         .         .       g.13921
 tcctgacttccctggaggagaagtattcctgttgctgtcttcagtctcacagagaagctc       c.*120

          .         .         .         .         .         .       g.13981
 ctgctacttccccagctctcagtagtttagtacaataatctctatttgagaagttctcag       c.*180

          .         .         .         .         .         .       g.14041
 aataactacctcctcacttggctgtctgaccagagaatgcacctcttgttactccctgtt       c.*240

          .         .         .         .         .         .       g.14101
 atttttctgccctgggttcttccacagcacaaacacacctctgccaccccaggtttttca       c.*300

          .         .         .         .         .         .       g.14161
 tctgaaaagcagttcatgtctgaaacagagaaccaaaccgcaaacgtgaaattctattga       c.*360

          .         .         .         .         .         .       g.14221
 aaacagtgtcttgagctctaaagtagcaactgctggtgattttttttttctttttactgt       c.*420

          .         .         .         .         .         .       g.14281
 tgaacttagaactatgctaatttttggagaaatgtcataaattactgttttgccaagaat       c.*480

          .         .         .         .         .         .       g.14341
 atagttattattgctgtttggtttgtttataatgttatcggctctattctctaaactggc       c.*540

          .         .         .         .         .         .       g.14401
 atctgctctagattcataaatacaaaaatgaatactgaattttgagtctatcctagtctt       c.*600

          .         .         .         .         .         .       g.14461
 cacaactttgacgtaattaaatccaactttcacagtgaagtgcctttttcctagaagtgg       c.*660

          .         .         .         .         .         .       g.14521
 tttgtagacttcctttataatatttcagtggaatagatgtctcaaaaatccttatgcatg       c.*720

          .         .         .         .         .         .       g.14581
 aaatgaatgtctgagatacgtctgtgacttatctaccattgaaggaaagctatatctatt       c.*780

          .         .         .         .         .         .       g.14641
 tgagagcagatgccattttgtacatgtatgaaattggttttccagaggcctgttttgggg       c.*840

          .         .         .         .         .         .       g.14701
 ctttcccaggagaaagatgaaactgaaagcacatgaataatttcacttaataatttttac       c.*900

          .         .         .         .         .         .       g.14761
 ctaatctccacttttttcataggttactacctatacaatgtatgtaatttgtttccccta       c.*960

          .         .         .         .         .         .       g.14821
 gcttactgataaacctaatattcaatgaacttccatttgtattcaaatttgtgtcatacc       c.*1020

          .         .         .         .         .         .       g.14881
 agaaagctctacatttgcagatgttcaaatattgtaaaactttggtgcattgttatttaa       c.*1080

          .         .         .         .         .         .       g.14941
 tagctgtgatcagtgattttcaaacctcaaatatagtatattaacaaattacattttcac       c.*1140

          .         .         .         .         .         .       g.15001
 tgtatatcatggtatcttaatgatgtatataattgccttcaatccccttctcaccccacc       c.*1200

          .         .         .         .         .         .       g.15061
 ctctacagcttcccccacagcaataggggcttgattatttcagttgagtaaagcatggtg       c.*1260

          .         .         .         .         .         .       g.15121
 ctaatggaccagggtcacagtttcaaaacttgaacaatccagttagcatcacagagaaag       c.*1320

          .         .         .         .         .         .       g.15181
 aaattcttctgcatttgctcattgcaccagtaactccagctagtaattttgctaggtagc       c.*1380

          .         .         .         .         .         .       g.15241
 tgcagttagccctgcaaggaaagaagaggtcagttagcacaaaccctttaccatgactgg       c.*1440

          .         .         .         .         .         .       g.15301
 aaaactcagtatcacgtatttaaacatttttttttcttttagccatgtagaaactctaaa       c.*1500

          .         .         .         .         .         .       g.15361
 ttaagccaatattctcatttgagaatgaggatgtctcagctgagaaacgttttaaattct       c.*1560

          .         .         .         .         .         .       g.15421
 ctttattcataatgttctttgaagggtttaaaacaagatgttgataaatctaagctgatg       c.*1620

          .         .         .         .         .         .       g.15481
 agtttgctcaaaacaggaagttgaaattgttgagacaggaatggaaaatataattaattg       c.*1680

          .         .         .         .         .         .       g.15541
 atacctatgaggatttggaggcttggcattttaatttgcagataataccctggtaattct       c.*1740

          .         .         .         .         .         .       g.15601
 catgaaaaatagacttggataacttttgataaaagactaattccaaaatggccactttgt       c.*1800

          .         .         .         .         .         .       g.15661
 tcctgtctttaatatctaaatacttactgaggtcctccatcttctatattatgaattttc       c.*1860

          .         .         .         .         .         .       g.15721
 atttattaagcaaatgtcatattaccttgaaattcagaagagaagaaacatatactgtgt       c.*1920

          .         .         .         .         .         .       g.15781
 ccagagtataatgaacctgcagagttgtgcttcttactgctaattctgggagctttcaca       c.*1980

          .         .         .         .         .         .       g.15841
 gtactgtcatcatttgtaaatggaaattctgcttttctgtttctgctccttctggagcag       c.*2040

          .         .         .         .         .         .       g.15901
 tgctactctgtaattttcctgaggcttatcacctcagtcatttcttttttaaatgtctgt       c.*2100

          .         .         .         .         .         .       g.15961
 gactggcagtgattctttttcttaaaaatctattaaatttgatgtcaaattagggagaaa       c.*2160

          .         .         .         .         .         .       g.16021
 gatagttactcatcttgggctcttgtgccaatagcccttgtatgtatgtacttagagttt       c.*2220

          .         .         .         .         .         .       g.16081
 tccaagtatgttctaagcacagaagtttctaaatggggccaaaattcagacttgagtatg       c.*2280

          .         .         .         .         .         .       g.16141
 ttctttgaataccttaagaagttacaattagccgggcatggtggcccgtgcctgtagtcc       c.*2340

          .         .         .         .         .         .       g.16201
 cagctacttgagaggctgaggcaggagaatcacttcaacccaggaggtggaggttacagt       c.*2400

          .         .         .         .         .         .       g.16261
 gagcagagatcgtgccactgcactccagcctgggtgacaagagagacttgtctccaaaaa       c.*2460

          .         .         .         .         .         .       g.16321
 aaaagttacacctaggtgtgaattttggcacaaaggagtgacaaacttatagttaaaagc       c.*2520

          .         .         .         .         .         .       g.16381
 tgaataacttcagtgtggtataaaacgtggtttttaggctatgtttgtgattgctgaaaa       c.*2580

          .         .         .         .         .         .       g.16441
 gaattctagtttacctcaaaatccttctctttccccaaattaagtgcctggccagctgtc       c.*2640

          .         .         .         .         .         .       g.16501
 ataaattacatattccttttggtttttttaaaggttacatgttcaagagtgaaaataaga       c.*2700

          .         .         .         .         .         .       g.16561
 tgttctgtctgaaggctaccatgccggatctgtaaatgaacctgttaaatgctgtatttg       c.*2760

          .         .         .         .         .         .       g.16621
 ctccaacggcttactatagaatgttacttaatacaatatcatacttattacaatttttac       c.*2820

          .         .         .         .         .         .       g.16681
 tataggagtgtaataggtaaaattaatctctattttagtgggcccatgtttagtctttca       c.*2880

          .         .         .         .         .         .       g.16741
 ccatcctttaaactgctgtgaatttttttgtcatgacttgaaagcaaggatagagaaaca       c.*2940

          .         .         .         .         .         .       g.16801
 ctttagagatatgtggggtttttttaccattccagagcttgtgagcataatcatatttgc       c.*3000

          .         .         .         .         .         .       g.16861
 tttatatttatagtcatgaactcctaagttggcagctacaaccaagaaccaaaaaatggt       c.*3060

          .         .         .         .         .         .       g.16921
 gcgttctgcttcttgtaattcatctctgctaataaattataagaagcaaggaaaattagg       c.*3120

          .         .         .         .         .         .       g.16981
 gaaaatattttatttggatggtttctataaacaagggactataattcttgtacattattt       c.*3180

          .         .         .         .         .         .       g.17041
 ttcatctttgctgtttctttgagcagtctaatgtgccacacaattatctaaggtatttgt       c.*3240

          .         .         .         .         .         .       g.17101
 tttctataagaattgttttaaaagtattcttgttaccagagtagttgtattatatttcaa       c.*3300

          .         .         .         .         .         .       g.17161
 aacgtaagatgatttttaaaagcctgagtactgacctaagatggaattgtatgaactctg       c.*3360

          .         .         .         .         .         .       g.17221
 ctctggagggaggggaggatgtccgtggaagttgtaagacttttatttttttgtgccatc       c.*3420

          .         .         .         .         .         .       g.17281
 aaatataggtaaaaataattgtgcaattctgctgtttaaacaggaactattggcctcctt       c.*3480

          .         .         .         .         .         .       g.17341
 ggccctaaatggaagggccgatattttaagttgattattttattgtaaattaatccaacc       c.*3540

          .         .         .         .         .         .       g.17401
 tagttctttttaatttggttgaatgttttttcttgttaaatgatgtttaaaaaataaaaa       c.*3600

          .         .         .                                     g.17431
 ctggaagttcttggcttagtcataattctt                                     c.*3630

 (downstream sequence)

Legend:
Nucleotide numbering (following the rules of the HGVS for a 'Coding DNA Reference Sequence') is indicated at the right of the sequence, counting the A of the ATG translation initiating Methionine as 1. Every 10^th nucleotide is indicated by a "." above the sequence. The Neuroblastoma RAS viral (v-ras) oncogene homolog protein sequence is shown below the coding DNA sequence, with numbering indicated at the right starting with 1 for the translation initiating Methionine. Every 10^th amino acid is shown in bold. The position of introns is indicated by a vertical line, splitting the two exons. The start of the first exon (transcription initiation site) is indicated by a '\', the end of the last exon (poly-A addition site) by a '/'. The exon number is indicated above the first nucleotide(s) of the exon. To aid the description of frame shift variants, all stop codons in the +1 frame are shown in bold while all stop codons in the +2 frame are underlined.