ribose 5-phosphate isomerase A (RPIA) - coding DNA reference sequence

(used for variant description)

(last modified August 25, 2020)


This file was created to facilitate the description of sequence variants on transcript NM_144563.2 in the RPIA gene based on a coding DNA reference sequence following the HGVS recommendations.

The sequence was taken from NG_016710.1, covering RPIA transcript NM_144563.2.


Please note that introns are available by clicking on the exon numbers above the sequence.
 (upstream sequence)
                     .         .         .         .                g.5041
                    cgggggcgggacttcagcggaggccggagcgaggcgtcggg       c.-1

          .         .         .         .         .         .       g.5101
 ATGCAGCGCCCCGGGCCCTTCAGCACCCTCTACGGGCGGGTCTTGGCCCCGCTGCCCGGG       c.60
 M  Q  R  P  G  P  F  S  T  L  Y  G  R  V  L  A  P  L  P  G         p.20

          .         .         .         .         .         .       g.5161
 AGGGCCGGGGGCGCGGCCTCCGGCGGAGGAGGGAACAGCTGGGACCTCCCGGGTTCCCAC       c.120
 R  A  G  G  A  A  S  G  G  G  G  N  S  W  D  L  P  G  S  H         p.40

          .         .         .         .         .         .       g.5221
 GTGCGGCTGCCGGGGCGTGCACAGTCTGGGACCCGTGGCGGTGCTGGCAACACAAGCACC       c.180
 V  R  L  P  G  R  A  Q  S  G  T  R  G  G  A  G  N  T  S  T         p.60

          .         .         .         .         .         .       g.5281
 AGCTGCGGGGACTCCAACAGCATCTGCCCGGCCCCCTCCACGATGTCCAAGGCCGAGGAG       c.240
 S  C  G  D  S  N  S  I  C  P  A  P  S  T  M  S  K  A  E  E         p.80

          .         .         .         .      | 02  .         .    g.11841
 GCCAAGAAGCTGGCGGGCCGCGCGGCTGTGGAGAACCACGTGAGG | AATAACCAAGTGCTG    c.300
 A  K  K  L  A  G  R  A  A  V  E  N  H  V  R   | N  N  Q  V  L      p.100

          .         .         .         .       | 03 .         .    g.13365
 GGAATTGGAAGTGGTTCTACAATTGTCCATGCTGTGCAGCGAATAG | CTGAAAGGGTGAAG    c.360
 G  I  G  S  G  S  T  I  V  H  A  V  Q  R  I  A |   E  R  V  K      p.120

          .         .         .         .   | 04     .         .    g.42638
 CAAGAGAATCTGAACCTCGTCTGTATTCCCACTTCCTTCCAG | GCCCGCCAGCTCATCCTG    c.420
 Q  E  N  L  N  L  V  C  I  P  T  S  F  Q   | A  R  Q  L  I  L      p.140

          .         .         .         .   | 05     .         .    g.47911
 CAGTATGGCTTGACCCTCAGTGATCTGGATCGACACCCAGAG | ATCGACCTTGCCATCGAT    c.480
 Q  Y  G  L  T  L  S  D  L  D  R  H  P  E   | I  D  L  A  I  D      p.160

          .         .         .         .        | 06.         .    g.49023
 GGTGCTGATGAAGTAGATGCTGATCTCAATCTCATCAAGGGTGGCGG | AGGCTGCCTGACC    c.540
 G  A  D  E  V  D  A  D  L  N  L  I  K  G  G  G  |  G  C  L  T      p.180

          .         .         .         .         .       | 07 .    g.49880
 CAGGAGAAGATTGTGGCTGGCTATGCTAGTCGCTTCATCGTGATCGCTGATTTCAG | GAAA    c.600
 Q  E  K  I  V  A  G  Y  A  S  R  F  I  V  I  A  D  F  R  |  K      p.200

          .         .         .         .         .         .       g.49940
 GATTCGAAGAATCTCGGGGATCAGTGGCACAAGGGAATCCCCATCGAGGTCATCCCAATG       c.660
 D  S  K  N  L  G  D  Q  W  H  K  G  I  P  I  E  V  I  P  M         p.220

          .         .         .         .         .         .       g.50000
 GCCTATGTCCCAGTGAGCCGAGCTGTGAGCCAGAAGTTTGGGGGCGTGGTTGAACTTCGA       c.720
 A  Y  V  P  V  S  R  A  V  S  Q  K  F  G  G  V  V  E  L  R         p.240

          .         | 08         .         .         .         .    g.51360
 ATGGCTGTCAACAAGGCT | GGTCCTGTGGTGACAGATAATGGGAATTTTATCTTGGACTGG    c.780
 M  A  V  N  K  A   | G  P  V  V  T  D  N  G  N  F  I  L  D  W      p.260

          .         .         .         .         .         | 09    g.63324
 AAGTTTGACCGGGTACACAAATGGAGTGAAGTGAATACAGCTATCAAAATGATCCCAG | GT    c.840
 K  F  D  R  V  H  K  W  S  E  V  N  T  A  I  K  M  I  P  G |       p.280

          .         .         .         .         .         .       g.63384
 GTGGTGGACACAGGCCTATTCATCAACATGGCTGAGAGAGTCTACTTTGGGATGCAGGAT       c.900
 V  V  D  T  G  L  F  I  N  M  A  E  R  V  Y  F  G  M  Q  D         p.300

          .         .         .                                     g.63420
 GGCTCAGTGAACATGAGGGAGAAGCCTTTCTGTTGA                               c.936
 G  S  V  N  M  R  E  K  P  F  C  X                                 p.311

          .         .         .         .         .         .       g.63480
 ccctgcaaggagcagagtgtgttcaccttgagtctccagcccacagccaaggtggacgta       c.*60

          .         .         .         .         .         .       g.63540
 cctctccaggagcctttgccttaatgtatctctgcctggacaacttgtggtggggggtgg       c.*120

          .         .         .         .         .         .       g.63600
 ggggaagagtgggagggggagttaaatccagtcttatgaagtattgttattaaatgtctt       c.*180

          .         .         .         .         .         .       g.63660
 tttaaaaagagaaatataaacatatatttttactattaaaatattcagttttttaaatga       c.*240

          .         .         .         .         .         .       g.63720
 agtagaacttgagttcatgttttatatgaaatatttaccaaaaaaaaaaaatgaggtaaa       c.*300

          .         .         .         .         .         .       g.63780
 ctgtatttaaaacctttgacttgagtctgctggtaaagcttctgaatattgagtttgctg       c.*360

          .         .         .         .         .         .       g.63840
 agaaataaaaatcaaaacttctttaagctggtaaagtgaggggcccaccagcagtgatct       c.*420

          .         .         .         .         .         .       g.63900
 cctgatgccttactggaaactttgtttacttgtctgctaccctctgatttgtttttagtt       c.*480

          .         .         .         .         .         .       g.63960
 agtttttattgtgagcacacatagtacctagttacatcttaagatcaggtttataaaact       c.*540

          .         .         .         .         .         .       g.64020
 gtggagtggagcggtatggtatggaatgacttggaatgtaagctgtcagggagaaaatgt       c.*600

          .         .         .         .         .         .       g.64080
 tgttacacttttgctaagatctgggggtttcttcatattcctgctgttggaagcagttga       c.*660

          .         .         .         .         .         .       g.64140
 ccagaaatgcttgccagtactgccaaagcactgctgtgaaatgtgaagtactttgttttt       c.*720

          .         .         .         .         .         .       g.64200
 ttatttttaatgattttctttttgttattaatatttttctctgttcctttgttattactt       c.*780

          .         .         .         .         .         .       g.64260
 gcatggtttggcgtcagaagtccttacctctttatattgtttgcaggtttaaataaaaca       c.*840

          .                                                         g.64277
 gtgtggtgccattttga                                                  c.*857

 (downstream sequence)
Legend:
Nucleotide numbering (following the rules of the HGVS for a 'Coding DNA Reference Sequence') is indicated at the right of the sequence, counting the A of the ATG translation initiating Methionine as 1. Every 10th nucleotide is indicated by a "." above the sequence. The Ribose 5-phosphate isomerase A protein sequence is shown below the coding DNA sequence, with numbering indicated at the right starting with 1 for the translation initiating Methionine. Every 10th amino acid is shown in bold. The position of introns is indicated by a vertical line, splitting the two exons. The start of the first exon (transcription initiation site) is indicated by a '\', the end of the last exon (poly-A addition site) by a '/'. The exon number is indicated above the first nucleotide(s) of the exon. To aid the description of frame shift variants, all stop codons in the +1 frame are shown in bold while all stop codons in the +2 frame are underlined.

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