SRY (sex determining region Y)-box 9 (SOX9) - coding DNA reference sequence

(used for variant description)

(last modified February 3, 2016)


This file was created to facilitate the description of sequence variants on transcript NM_000346.3 in the SOX9 gene based on a coding DNA reference sequence following the HGVS recommendations.

The sequence was taken from NG_012490.1, covering SOX9 transcript NM_000346.3.


Please note that introns are available by clicking on the exon numbers above the sequence.
 (upstream sequence)
                                                   .                g.5012
                                                 ggagagccgaaa       c.-361

 .         .         .         .         .         .                g.5072
 gcggagctcgaaactgactggaaacttcagtggcgcggagactcgccagtttcaaccccg       c.-301

 .         .         .         .         .         .                g.5132
 gaaacttttctttgcaggaggagaagagaaggggtgcaagcgcccccacttttgctcttt       c.-241

 .         .         .         .         .         .                g.5192
 ttcctcccctcctcctcctctccaattcgcctccccccacttggagcgggcagctgtgaa       c.-181

 .         .         .         .         .         .                g.5252
 ctggccaccccgcgccttcctaagtgctcgccgcggtagccggccgacgcgccagcttcc       c.-121

 .         .         .         .         .         .                g.5312
 ccgggagccgcttgctccgcatccgggcagccgaggggagaggagcccgcgcctcgagtc       c.-61

 .         .         .         .         .         .                g.5372
 cccgagccgccgcggcttctcgcctttcccggccaccagccccctgccccgggcccgcgt       c.-1

          .         .         .         .         .         .       g.5432
 ATGAATCTCCTGGACCCCTTCATGAAGATGACCGACGAGCAGGAGAAGGGCCTGTCCGGC       c.60
 M  N  L  L  D  P  F  M  K  M  T  D  E  Q  E  K  G  L  S  G         p.20

          .         .         .         .         .         .       g.5492
 GCCCCCAGCCCCACCATGTCCGAGGACTCCGCGGGCTCGCCCTGCCCGTCGGGCTCCGGC       c.120
 A  P  S  P  T  M  S  E  D  S  A  G  S  P  C  P  S  G  S  G         p.40

          .         .         .         .         .         .       g.5552
 TCGGACACCGAGAACACGCGGCCCCAGGAGAACACGTTCCCCAAGGGCGAGCCCGATCTG       c.180
 S  D  T  E  N  T  R  P  Q  E  N  T  F  P  K  G  E  P  D  L         p.60

          .         .         .         .         .         .       g.5612
 AAGAAGGAGAGCGAGGAGGACAAGTTCCCCGTGTGCATCCGCGAGGCGGTCAGCCAGGTG       c.240
 K  K  E  S  E  E  D  K  F  P  V  C  I  R  E  A  V  S  Q  V         p.80

          .         .         .         .         .         .       g.5672
 CTCAAAGGCTACGACTGGACGCTGGTGCCCATGCCGGTGCGCGTCAACGGCTCCAGCAAG       c.300
 L  K  G  Y  D  W  T  L  V  P  M  P  V  R  V  N  G  S  S  K         p.100

          .         .         .         .         .         .       g.5732
 AACAAGCCGCACGTCAAGCGGCCCATGAACGCCTTCATGGTGTGGGCGCAGGCGGCGCGC       c.360
 N  K  P  H  V  K  R  P  M  N  A  F  M  V  W  A  Q  A  A  R         p.120

          .         .         .         .         .         .       g.5792
 AGGAAGCTCGCGGACCAGTACCCGCACTTGCACAACGCCGAGCTCAGCAAGACGCTGGGC       c.420
 R  K  L  A  D  Q  Y  P  H  L  H  N  A  E  L  S  K  T  L  G         p.140

          .  | 02      .         .         .         .         .    g.6748
 AAGCTCTGGAG | ACTTCTGAACGAGAGCGAGAAGCGGCCCTTCGTGGAGGAGGCGGAGCGG    c.480
 K  L  W  R  |  L  L  N  E  S  E  K  R  P  F  V  E  E  A  E  R      p.160

          .         .         .         .         .         .       g.6808
 CTGCGCGTGCAGCACAAGAAGGACCACCCGGATTACAAGTACCAGCCGCGGCGGAGGAAG       c.540
 L  R  V  Q  H  K  K  D  H  P  D  Y  K  Y  Q  P  R  R  R  K         p.180

          .         .         .         .         .         .       g.6868
 TCGGTGAAGAACGGGCAGGCGGAGGCAGAGGAGGCCACGGAGCAGACGCACATCTCCCCC       c.600
 S  V  K  N  G  Q  A  E  A  E  E  A  T  E  Q  T  H  I  S  P         p.200

          .         .         .         .         .         .       g.6928
 AACGCCATCTTCAAGGCGCTGCAGGCCGACTCGCCACACTCCTCCTCCGGCATGAGCGAG       c.660
 N  A  I  F  K  A  L  Q  A  D  S  P  H  S  S  S  G  M  S  E         p.220

          .         .      | 03  .         .         .         .    g.7558
 GTGCACTCCCCCGGCGAGCACTCGG | GGCAATCCCAGGGCCCACCGACCCCACCCACCACC    c.720
 V  H  S  P  G  E  H  S  G |   Q  S  Q  G  P  P  T  P  P  T  T      p.240

          .         .         .         .         .         .       g.7618
 CCCAAAACCGACGTGCAGCCGGGCAAGGCTGACCTGAAGCGAGAGGGGCGCCCCTTGCCA       c.780
 P  K  T  D  V  Q  P  G  K  A  D  L  K  R  E  G  R  P  L  P         p.260

          .         .         .         .         .         .       g.7678
 GAGGGGGGCAGACAGCCCCCTATCGACTTCCGCGACGTGGACATCGGCGAGCTGAGCAGC       c.840
 E  G  G  R  Q  P  P  I  D  F  R  D  V  D  I  G  E  L  S  S         p.280

          .         .         .         .         .         .       g.7738
 GACGTCATCTCCAACATCGAGACCTTCGATGTCAACGAGTTTGACCAGTACCTGCCGCCC       c.900
 D  V  I  S  N  I  E  T  F  D  V  N  E  F  D  Q  Y  L  P  P         p.300

          .         .         .         .         .         .       g.7798
 AACGGCCACCCGGGGGTGCCGGCCACGCACGGCCAGGTCACCTACACGGGCAGCTACGGC       c.960
 N  G  H  P  G  V  P  A  T  H  G  Q  V  T  Y  T  G  S  Y  G         p.320

          .         .         .         .         .         .       g.7858
 ATCAGCAGCACCGCGGCCACCCCGGCGAGCGCGGGCCACGTGTGGATGTCCAAGCAGCAG       c.1020
 I  S  S  T  A  A  T  P  A  S  A  G  H  V  W  M  S  K  Q  Q         p.340

          .         .         .         .         .         .       g.7918
 GCGCCGCCGCCACCCCCGCAGCAGCCCCCACAGGCCCCGCCGGCCCCGCAGGCGCCCCCG       c.1080
 A  P  P  P  P  P  Q  Q  P  P  Q  A  P  P  A  P  Q  A  P  P         p.360

          .         .         .         .         .         .       g.7978
 CAGCCGCAGGCGGCGCCCCCACAGCAGCCGGCGGCACCCCCGCAGCAGCCACAGGCGCAC       c.1140
 Q  P  Q  A  A  P  P  Q  Q  P  A  A  P  P  Q  Q  P  Q  A  H         p.380

          .         .         .         .         .         .       g.8038
 ACGCTGACCACGCTGAGCAGCGAGCCGGGCCAGTCCCAGCGAACGCACATCAAGACGGAG       c.1200
 T  L  T  T  L  S  S  E  P  G  Q  S  Q  R  T  H  I  K  T  E         p.400

          .         .         .         .         .         .       g.8098
 CAGCTGAGCCCCAGCCACTACAGCGAGCAGCAGCAGCACTCGCCCCAACAGATCGCCTAC       c.1260
 Q  L  S  P  S  H  Y  S  E  Q  Q  Q  H  S  P  Q  Q  I  A  Y         p.420

          .         .         .         .         .         .       g.8158
 AGCCCCTTCAACCTCCCACACTACAGCCCCTCCTACCCGCCCATCACCCGCTCACAGTAC       c.1320
 S  P  F  N  L  P  H  Y  S  P  S  Y  P  P  I  T  R  S  Q  Y         p.440

          .         .         .         .         .         .       g.8218
 GACTACACCGACCACCAGAACTCCAGCTCCTACTACAGCCACGCGGCAGGCCAGGGCACC       c.1380
 D  Y  T  D  H  Q  N  S  S  S  Y  Y  S  H  A  A  G  Q  G  T         p.460

          .         .         .         .         .         .       g.8278
 GGCCTCTACTCCACCTTCACCTACATGAACCCCGCTCAGCGCCCCATGTACACCCCCATC       c.1440
 G  L  Y  S  T  F  T  Y  M  N  P  A  Q  R  P  M  Y  T  P  I         p.480

          .         .         .         .         .         .       g.8338
 GCCGACACCTCTGGGGTCCCTTCCATCCCGCAGACCCACAGCCCCCAGCACTGGGAACAA       c.1500
 A  D  T  S  G  V  P  S  I  P  Q  T  H  S  P  Q  H  W  E  Q         p.500

          .         .         .                                     g.8368
 CCCGTCTACACACAGCTCACTCGACCTTGA                                     c.1530
 P  V  Y  T  Q  L  T  R  P  X                                       p.509

          .         .         .         .         .         .       g.8428
 ggaggcctcccacgaagggcgaagatggccgagatgatcctaaaaataaccgaagaaaga       c.*60

          .         .         .         .         .         .       g.8488
 gaggaccaaccagaattccctttggacatttgtgtttttttgtttttttattttgttttg       c.*120

          .         .         .         .         .         .       g.8548
 ttttttcttcttcttcttcttccttaaagacatttaagctaaaggcaactcgtacccaaa       c.*180

          .         .         .         .         .         .       g.8608
 tttccaagacacaaacatgacctatccaagcgcattacccacttgtggccaatcagtggc       c.*240

          .         .         .         .         .         .       g.8668
 caggccaaccttggctaaatggagcagcgaaatcaacgagaaactggactttttaaaccc       c.*300

          .         .         .         .         .         .       g.8728
 tcttcagagcaagcgtggaggatgatggagaatcgtgtgatcagtgtgctaaatctctct       c.*360

          .         .         .         .         .         .       g.8788
 gcctgtttggactttgtaattatttttttagcagtaattaaagaaaaaagtcctctgtga       c.*420

          .         .         .         .         .         .       g.8848
 ggaatattctctattttaaatatttttagtatgtactgtgtatgattcattaccattttg       c.*480

          .         .         .         .         .         .       g.8908
 aggggatttatacatatttttagataaaattaaatgctcttatttttccaacagctaaac       c.*540

          .         .         .         .         .         .       g.8968
 tactcttagttgaacagtgtgccctagcttttcttgcaaccagagtatttttgtacagat       c.*600

          .         .         .         .         .         .       g.9028
 ttgctttctcttacaaaaagaaaaaaaaaatcctgttgtattaacatttaaaaacagaat       c.*660

          .         .         .         .         .         .       g.9088
 tgtgttatgtgatcagttttgggggttaactttgcttaattcctcaggctttgcgattta       c.*720

          .         .         .         .         .         .       g.9148
 aggaggagctgccttaaaaaaaaataaaggccttattttgcaattatgggagtaaacaat       c.*780

          .         .         .         .         .         .       g.9208
 agtctagagaagcatttggtaagctttatcatatatatattttttaaagaagagaaaaac       c.*840

          .         .         .         .         .         .       g.9268
 accttgagccttaaaacggtgctgctgggaaacatttgcactcttttagtgcatttcctc       c.*900

          .         .         .         .         .         .       g.9328
 ctgcctttgcttgttcactgcagtcttaagaaagaggtaaaaggcaagcaaaggagatga       c.*960

          .         .         .         .         .         .       g.9388
 aatctgttctgggaatgtttcagcagccaataagtgcccgagcacactgcccccggttgc       c.*1020

          .         .         .         .         .         .       g.9448
 ctgcctgggccccatgtggaaggcagatgcctgctcgctctgtcacctgtgcctctcaga       c.*1080

          .         .         .         .         .         .       g.9508
 acaccagcagttaaccttcaagacattccacttgctaaaattatttattttgtaaggaga       c.*1140

          .         .         .         .         .         .       g.9568
 ggttttaattaaaacaaaaaaaaattcttttttttttttttttccaattttaccttcttt       c.*1200

          .         .         .         .         .         .       g.9628
 aaaataggttgttggagctttcctcaaagggtatggtcatctgttgttaaattatgttct       c.*1260

          .         .         .         .         .         .       g.9688
 taactgtaaccagtttttttttatttatctctttaatctttttttattattaaaagcaag       c.*1320

          .         .         .         .         .         .       g.9748
 tttctttgtattcctcaccctagatttgtataaatgcctttttgtccatcccttttttct       c.*1380

          .         .         .         .         .         .       g.9808
 ttgttgtttttgttgaaaacaaactggaaacttgtttctttttttgtataaatgagagat       c.*1440

          .         .         .         .         .         .       g.9868
 tgcaaatgtagtgtatcactgagtcatttgcagtgttttctgccacagacctttgggctg       c.*1500

          .         .         .         .         .         .       g.9928
 ccttatattgtgtgtgtgtgtgggtgtgtgtgtgttttgacacaaaaacaatgcaagcat       c.*1560

          .         .         .         .         .         .       g.9988
 gtgtcatccatatttctctgcatcttctcttggagtgagggaggctacctggaggggatc       c.*1620

          .         .         .         .         .         .       g.10048
 agcccactgacagaccttaatcttaattactgctgtggctagagagtttgaggattgctt       c.*1680

          .         .         .         .         .         .       g.10108
 tttaaaaaagacagcaaacttttttttttatttaaaaaaagatatattaacagttttaga       c.*1740

          .         .         .         .         .         .       g.10168
 agtcagtagaataaaatcttaaagcactcataatatggcatccttcaatttctgtataaa       c.*1800

          .         .         .         .         .         .       g.10228
 agcagatctttttaaaaagatacttctgtaacttaagaaacctggcatttaaatcatatt       c.*1860

          .         .         .         .         .         .       g.10288
 ttgtctttaggtaaaagctttggtttgtgttcgtgttttgtttgtttcacttgtttccct       c.*1920

          .         .         .         .         .         .       g.10348
 cccagccccaaaccttttgttctctccgtgaaacttacctttccctttttctttctcttt       c.*1980

          .         .         .         .         .                 g.10401
 tttttttttgtatattattgtttacaataaatatacattgcattaaaaagaaa              c.*2033

 (downstream sequence)
Legend:
Nucleotide numbering (following the rules of the HGVS for a 'Coding DNA Reference Sequence') is indicated at the right of the sequence, counting the A of the ATG translation initiating Methionine as 1. Every 10th nucleotide is indicated by a "." above the sequence. The SRY (sex determining region Y)-box 9 protein sequence is shown below the coding DNA sequence, with numbering indicated at the right starting with 1 for the translation initiating Methionine. Every 10th amino acid is shown in bold. The position of introns is indicated by a vertical line, splitting the two exons. The start of the first exon (transcription initiation site) is indicated by a '\', the end of the last exon (poly-A addition site) by a '/'. The exon number is indicated above the first nucleotide(s) of the exon. To aid the description of frame shift variants, all stop codons in the +1 frame are shown in bold while all stop codons in the +2 frame are underlined.

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