secreted protein, acidic, cysteine-rich (osteonectin) (SPARC) - coding DNA reference sequence

(used for variant description)

(last modified May 16, 2021)

This file was created to facilitate the description of sequence variants on transcript NM_003118.3 in the SPARC gene based on a coding DNA reference sequence following the HGVS recommendations.

The sequence was taken from NC_000005.9, covering SPARC transcript NM_003118.3.

Please note that introns are available by clicking on the exon numbers above the sequence.

 (upstream sequence)
                                         .         .                g.5023
                                      gggagaaggaggaggccggggga       c.-181

 .         .         .         .         .         .                g.5083
 aggaggagacaggaggaggagggaccacggggtggaggggagatagacccagcccagagc       c.-121

 .         .         .         .         .         .                g.5143
 tctgagtggtttcctgttgcctgtctctaaacccctccacattcccgcggtccttcagac       c.-61

 .         .         .         .         .       | 02 .             g.15866
 tgcccggagagcgcgctctgcctgccgcctgcctgcctgccactgag | ggttcccagcacc    c.-1

          .         .         .         .         .        | 03.    g.17383
 ATGAGGGCCTGGATCTTCTTTCTCCTTTGCCTGGCCGGGAGGGCCTTGGCAGCCCCT | CAG    c.60
 M  R  A  W  I  F  F  L  L  C  L  A  G  R  A  L  A  A  P   | Q      p.20

          .         .         .         .         .         .       g.17443
 CAAGAAGCCCTGCCTGATGAGACAGAGGTGGTGGAAGAAACTGTGGCAGAGGTGACTGAG       c.120
 Q  E  A  L  P  D  E  T  E  V  V  E  E  T  V  A  E  V  T  E         p.40

  | 04       .         .         .         .         .         .    g.18898
  | GTATCTGTGGGAGCTAATCCTGTCCAGGTGGAAGTAGGAGAATTTGATGATGGTGCAGAG    c.180
  | V  S  V  G  A  N  P  V  Q  V  E  V  G  E  F  D  D  G  A  E      p.60

          .         .         | 05         .         .         .    g.20392
 GAAACCGAAGAGGAGGTGGTGGCGGAAA | ATCCCTGCCAGAACCACCACTGCAAACACGGC    c.240
 E  T  E  E  E  V  V  A  E  N |   P  C  Q  N  H  H  C  K  H  G      p.80

          .         .         .         .         .         .       g.20452
 AAGGTGTGCGAGCTGGATGAGAACAACACCCCCATGTGCGTGTGCCAGGACCCCACCAGC       c.300
 K  V  C  E  L  D  E  N  N  T  P  M  C  V  C  Q  D  P  T  S         p.100

          .         .         . | 06       .         .         .    g.22300
 TGCCCAGCCCCCATTGGCGAGTTTGAGAAG | GTGTGCAGCAATGACAACAAGACCTTCGAC    c.360
 C  P  A  P  I  G  E  F  E  K   | V  C  S  N  D  N  K  T  F  D      p.120

          .         .         .         .         .         .       g.22360
 TCTTCCTGCCACTTCTTTGCCACAAAGTGCACCCTGGAGGGCACCAAGAAGGGCCACAAG       c.420
 S  S  C  H  F  F  A  T  K  C  T  L  E  G  T  K  K  G  H  K         p.140

          .         .         .  | 07      .         .         .    g.24483
 CTCCACCTGGACTACATCGGGCCTTGCAAAT | ACATCCCCCCTTGCCTGGACTCTGAGCTG    c.480
 L  H  L  D  Y  I  G  P  C  K  Y |   I  P  P  C  L  D  S  E  L      p.160

          .         .         .         .         .         .       g.24543
 ACCGAATTCCCCCTGCGCATGCGGGACTGGCTCAAGAACGTCCTGGTCACCCTGTATGAG       c.540
 T  E  F  P  L  R  M  R  D  W  L  K  N  V  L  V  T  L  Y  E         p.180

          .         .         .         .      | 08  .         .    g.25560
 AGGGATGAGGACAACAACCTTCTGACTGAGAAGCAGAAGCTGCGG | GTGAAGAAGATCCAT    c.600
 R  D  E  D  N  N  L  L  T  E  K  Q  K  L  R   | V  K  K  I  H      p.200

          .         .         .         .         .         .       g.25620
 GAGAATGAGAAGCGCCTGGAGGCAGGAGACCACCCCGTGGAGCTGCTGGCCCGGGACTTC       c.660
 E  N  E  K  R  L  E  A  G  D  H  P  V  E  L  L  A  R  D  F         p.220

          .         .         .         .         .         .       g.25680
 GAGAAGAACTATAACATGTACATCTTCCCTGTACACTGGCAGTTCGGCCAGCTGGACCAG       c.720
 E  K  N  Y  N  M  Y  I  F  P  V  H  W  Q  F  G  Q  L  D  Q         p.240

          .     | 09   .         .         .         .         .    g.27865
 CACCCCATTGACGG | GTACCTCTCCCACACCGAGCTGGCTCCACTGCGTGCTCCCCTCATC    c.780
 H  P  I  D  G  |  Y  L  S  H  T  E  L  A  P  L  R  A  P  L  I      p.260

          .         .         .         .         .         .       g.27925
 CCCATGGAGCATTGCACCACCCGCTTTTTCGAGACCTGTGACCTGGACAATGACAAGTAC       c.840
 P  M  E  H  C  T  T  R  F  F  E  T  C  D  L  D  N  D  K  Y         p.280

          .         .         .         .    | 10    .         .    g.28472
 ATCGCCCTGGATGAGTGGGCCGGCTGCTTCGGCATCAAGCAGA | AGGATATCGACAAGGAT    c.900
 I  A  L  D  E  W  A  G  C  F  G  I  K  Q  K |   D  I  D  K  D      p.300

          .                                                         g.28484
 CTTGTGATCTAA                                                       c.912
 L  V  I  X                                                         p.303

          .         .         .         .         .         .       g.28544
 atccactccttccacagtaccggattctctctttaaccctccccttcgtgtttcccccaa       c.*60

          .         .         .         .         .         .       g.28604
 tgtttaaaatgtttggatggtttgttgttctgcctggagacaaggtgctaacatagattt       c.*120

          .         .         .         .         .         .       g.28664
 aagtgaatacattaacggtgctaaaaatgaaaattctaacccaagacatgacattcttag       c.*180

          .         .         .         .         .         .       g.28724
 ctgtaacttaactattaaggccttttccacacgcattaatagtcccatttttctcttgcc       c.*240

          .         .         .         .         .         .       g.28784
 atttgtagctttgcccattgtcttattggcacatgggtggacacggatctgctgggctct       c.*300

          .         .         .         .         .         .       g.28844
 gccttaaacacacattgcagcttcaacttttctctttagtgttctgtttgaaactaatac       c.*360

          .         .         .         .         .         .       g.28904
 ttaccgagtcagactttgtgttcatttcatttcagggtcttggctgcctgtgggcttccc       c.*420

          .         .         .         .         .         .       g.28964
 caggtggcctggaggtgggcaaagggaagtaacagacacacgatgttgtcaaggatggtt       c.*480

          .         .         .         .         .         .       g.29024
 ttgggactagaggctcagtggtgggagagatccctgcagaacccaccaaccagaacgtgg       c.*540

          .         .         .         .         .         .       g.29084
 tttgcctgaggctgtaactgagagaaagattctggggctgtgttatgaaaatatagacat       c.*600

          .         .         .         .         .         .       g.29144
 tctcacataagcccagttcatcaccatttcctcctttacctttcagtgcagtttcttttc       c.*660

          .         .         .         .         .         .       g.29204
 acattaggctgttggttcaaacttttgggagcacggactgtcagttctctgggaagtggt       c.*720

          .         .         .         .         .         .       g.29264
 cagcgcatcctgcagggcttctcctcctctgtcttttggagaaccagggctcttctcagg       c.*780

          .         .         .         .         .         .       g.29324
 ggctctagggactgccaggctgtttcagccaggaaggccaaaatcaagagtgagatgtag       c.*840

          .         .         .         .         .         .       g.29384
 aaagttgtaaaatagaaaaagtggagttggtgaatcggttgttctttcctcacatttgga       c.*900

          .         .         .         .         .         .       g.29444
 tgattgtcataaggtttttagcatgttcctccttttcttcaccctccccttttttcttct       c.*960

          .         .         .         .         .         .       g.29504
 attaatcaagagaaacttcaaagttaatgggatggtcggatctcacaggctgagaactcg       c.*1020

          .         .         .         .         .         .       g.29564
 ttcacctccaagcatttcatgaaaaagctgcttcttattaatcatacaaactctcaccat       c.*1080

          .         .         .         .         .         .       g.29624
 gatgtgaagagtttcacaaatccttcaaaataaaaagtaatgacttagaaactgccttcc       c.*1140

          .         .         .         .         .         .       g.29684
 tgggtgatttgcatgtgtcttagtcttagtcaccttattatcctgacacaaaaacacatg       c.*1200

          .         .         .         .         .         .       g.29744
 agcatacatgtctacacatgactacacaaatgcaaacctttgcaaacacattatgctttt       c.*1260

          .         .         .         .         .         .       g.29804
 gcacacacacacctgtacacacacaccggcatgtttatacacagggagtgtatggttcct       c.*1320

          .         .         .         .         .         .       g.29864
 gtaagcactaagttagctgttttcatttaatgacctgtggtttaacccttttgatcacta       c.*1380

          .         .         .         .         .         .       g.29924
 ccaccattatcagcaccagactgagcagctatatccttttattaatcatggtcattcatt       c.*1440

          .         .         .         .         .         .       g.29984
 cattcattcattcacaaaatatttatgatgtatttactctgcaccaggtcccatgccaag       c.*1500

          .         .         .         .         .         .       g.30044
 cactggggacacagttatggcaaagtagacaaagcatttgttcatttggagcttagagtc       c.*1560

          .         .         .         .         .         .       g.30104
 caggaggaatacattagataatgacacaatcaaatataaattgcaagatgtcacaggtgt       c.*1620

          .         .         .         .         .         .       g.30164
 gatgaagggagagtaggagagaccatgagtatgtgtaacaggaggacacagcattattct       c.*1680

          .         .         .         .         .         .       g.30224
 agtgctgtactgttccgtacggcagccactacccacatgtaactttttaagatttaaatt       c.*1740

          .         .         .         .         .         .       g.30284
 taaattagttaacattcaaaacgcagctccccaatcacactagcaacatttcaagtgctt       c.*1800

          .         .         .         .         .         .       g.30344
 gagagccatgcatgattagtggttaccctattgaataggtcagaagtagaatcttttcat       c.*1860

          .         .         .         .         .         .       g.30404
 catcacagaaagttctattggacagtgctcttctagatcatcataagactacagagcact       c.*1920

          .         .         .         .         .         .       g.30464
 tttcaaagctcatgcatgttcatcatgttagtgtcgtattttgagctggggttttgagac       c.*1980

          .         .         .         .         .         .       g.30524
 tccccttagagatagagaaacagacccaagaaatgtgctcaattgcaatgggccacatac       c.*2040

          .         .         .         .         .         .       g.30584
 ctagatctccagatgtcatttcccctctcttattttaagttatgttaagattactaaaac       c.*2100

          .         .         .         .         .         .       g.30644
 aataaaagctcctaaaaaatcaaactgtattctggtgttctcttctacacagtgggaggg       c.*2160

          .         .         .         .         .         .       g.30704
 cgagcagtaggagagattggcccatttggtgctggccatttgaggaatgcaagcccagca       c.*2220

          .         .         .         .         .         .       g.30764
 ctagtctcataatctctaggaatctgtagagagaggaattgaagtaaatttcagcattgg       c.*2280

          .         .         .         .         .         .       g.30824
 ctcattcagtcattcggcgacattcatcaggtacctgcaatgtgttaggggatcttatga       c.*2340

          .         .         .         .         .         .       g.30884
 gtaggcagcgtgcgtgatccttgctcccctggagctttctaacattctagcaggcagacc       c.*2400

          .         .         .         .         .         .       g.30944
 acacataaatttgcaatactgtttctgataaaaacgtgctgtaaaggaaataaagcagag       c.*2460

          .                                                         g.30959
 aactatcatggaaaa                                                    c.*2475

 (downstream sequence)

Legend:
Nucleotide numbering (following the rules of the HGVS for a 'Coding DNA Reference Sequence') is indicated at the right of the sequence, counting the A of the ATG translation initiating Methionine as 1. Every 10^th nucleotide is indicated by a "." above the sequence. The Secreted protein, acidic, cysteine-rich (osteonectin) protein sequence is shown below the coding DNA sequence, with numbering indicated at the right starting with 1 for the translation initiating Methionine. Every 10^th amino acid is shown in bold. The position of introns is indicated by a vertical line, splitting the two exons. The start of the first exon (transcription initiation site) is indicated by a '\', the end of the last exon (poly-A addition site) by a '/'. The exon number is indicated above the first nucleotide(s) of the exon. To aid the description of frame shift variants, all stop codons in the +1 frame are shown in bold while all stop codons in the +2 frame are underlined.