sprouty homolog 2 (Drosophila) (SPRY2) - coding DNA reference sequence

(used for variant description)

(last modified December 12, 2014)

This file was created to facilitate the description of sequence variants on transcript NM_005842.2 in the SPRY2 gene based on a coding DNA reference sequence following the HGVS recommendations.

The sequence was taken from NC_000013.10, covering SPRY2 transcript NM_005842.2.

Please note that introns are available by clicking on the exon numbers above the sequence.

 (upstream sequence)
                                         .         .                g.5021
                                        gtaaggccgttttcttttccc       c.-361

 .         .         .         .         .         .                g.5081
 attcgctcatctgccaggaaaagggacttgccgttggcgcttcggcctcttgttcattga       c.-301

 .         .         .         .         .         .                g.5141
 gaaaaaagaggaaatactccgcgtgcgcttgtagaaggggagtcgtctccagctccgaac       c.-241

 .         .         .         .         .         .                g.5201
 cccggagtgttcatcagcggggaatctggctccgaattctctttttttctcccgccgatt       c.-181

 .         .         .         .         .         .                g.5261
 gctcggaagttggtctaaagcagaggttggaaagaaaggaaaaaagtttgcatcgagact       c.-121

 .         .         .         .         .         .                g.5321
 ggatttatttgcacatcgcagaaagaagagaatccaagggagaggggttggtgcaaagcc       c.-61

 .         | 02         .         .         .         .             g.8246
 gcgatcacg | gagttcagatgtgttctaagcctgctggagtgaccacacttccaagacctg    c.-1

          .         .         .         .         .         .       g.8306
 ATGGAGGCCAGAGCTCAGAGTGGCAACGGGTCGCAGCCCTTGCTGCAGACGCCCCGTGAC       c.60
 M  E  A  R  A  Q  S  G  N  G  S  Q  P  L  L  Q  T  P  R  D         p.20

          .         .         .         .         .         .       g.8366
 GGTGGCAGACAGCGTGGGGAGCCCGACCCCAGAGACGCCCTCACCCAGCAGGTACATGTC       c.120
 G  G  R  Q  R  G  E  P  D  P  R  D  A  L  T  Q  Q  V  H  V         p.40

          .         .         .         .         .         .       g.8426
 TTGTCTCTGGATCAGATCAGAGCCATCCGAAACACCAATGAGTACACAGAGGGGCCTACT       c.180
 L  S  L  D  Q  I  R  A  I  R  N  T  N  E  Y  T  E  G  P  T         p.60

          .         .         .         .         .         .       g.8486
 GTCGTCCCAAGACCTGGGCTCAAGCCTGCTCCTCGCCCCTCCACTCAGCACAAACACGAG       c.240
 V  V  P  R  P  G  L  K  P  A  P  R  P  S  T  Q  H  K  H  E         p.80

          .         .         .         .         .         .       g.8546
 AGACTCCACGGTCTGCCTGAGCACCGCCAGCCTCCTAGGCTCCAGCACTCGCAGGTCCAT       c.300
 R  L  H  G  L  P  E  H  R  Q  P  P  R  L  Q  H  S  Q  V  H         p.100

          .         .         .         .         .         .       g.8606
 TCTTCTGCACGAGCCCCTCTGTCCAGATCCATAAGCACGGTCAGCTCAGGGTCGCGGAGC       c.360
 S  S  A  R  A  P  L  S  R  S  I  S  T  V  S  S  G  S  R  S         p.120

          .         .         .         .         .         .       g.8666
 AGTACGAGGACAAGTACCAGCAGCAGCTCCTCTGAACAGAGACTGCTAGGATCATCCTTC       c.420
 S  T  R  T  S  T  S  S  S  S  S  E  Q  R  L  L  G  S  S  F         p.140

          .         .         .         .         .         .       g.8726
 TCCTCCGGGCCTGTTGCTGATGGCATAATCCGGGTGCAACCCAAATCTGAGCTCAAGCCA       c.480
 S  S  G  P  V  A  D  G  I  I  R  V  Q  P  K  S  E  L  K  P         p.160

          .         .         .         .         .         .       g.8786
 GGTGAGCTTAAGCCACTGAGCAAGGAAGATTTGGGCCTGCACGCCTACAGGTGTGAGGAC       c.540
 G  E  L  K  P  L  S  K  E  D  L  G  L  H  A  Y  R  C  E  D         p.180

          .         .         .         .         .         .       g.8846
 TGTGGCAAGTGCAAATGTAAGGAGTGCACCTACCCAAGGCCTCTGCCATCAGACTGGATC       c.600
 C  G  K  C  K  C  K  E  C  T  Y  P  R  P  L  P  S  D  W  I         p.200

          .         .         .         .         .         .       g.8906
 TGCGACAAGCAGTGCCTTTGCTCGGCCCAGAACGTGATTGACTATGGGACTTGTGTATGC       c.660
 C  D  K  Q  C  L  C  S  A  Q  N  V  I  D  Y  G  T  C  V  C         p.220

          .         .         .         .         .         .       g.8966
 TGTGTGAAAGGTCTCTTCTATCACTGTTCTAATGATGATGAGGACAACTGTGCTGACAAC       c.720
 C  V  K  G  L  F  Y  H  C  S  N  D  D  E  D  N  C  A  D  N         p.240

          .         .         .         .         .         .       g.9026
 CCATGTTCTTGCAGCCAGTCTCACTGTTGTACACGATGGTCAGCCATGGGTGTCATGTCC       c.780
 P  C  S  C  S  Q  S  H  C  C  T  R  W  S  A  M  G  V  M  S         p.260

          .         .         .         .         .         .       g.9086
 CTCTTTTTGCCTTGTTTATGGTGTTACCTTCCAGCCAAGGGTTGCCTTAAATTGTGCCAG       c.840
 L  F  L  P  C  L  W  C  Y  L  P  A  K  G  C  L  K  L  C  Q         p.280

          .         .         .         .         .         .       g.9146
 GGGTGTTATGACCGGGTTAACAGGCCTGGTTGCCGCTGTAAAAACTCAAACACAGTTTGC       c.900
 G  C  Y  D  R  V  N  R  P  G  C  R  C  K  N  S  N  T  V  C         p.300

          .         .         .         .                           g.9194
 TGCAAAGTTCCCACTGTCCCCCCTAGGAACTTTGAAAAACCAACATAG                   c.948
 C  K  V  P  T  V  P  P  R  N  F  E  K  P  T  X                     p.315

          .         .         .         .         .         .       g.9254
 catcattaatcaggaatattacagtaatgaggattttttctttctttttttaatacacat       c.*60

          .         .         .         .         .         .       g.9314
 atgcaaccaactaaacagttataatcttggcactgttaatagaaaggtgggatagtcttt       c.*120

          .         .         .         .         .         .       g.9374
 gctgtttgcggtgaaatgctttttgtccatgtgccgttttaactgatatgcttgttagaa       c.*180

          .         .         .         .         .         .       g.9434
 ctcagctaatggagctcaaagtatgagatacagaacttggtgacccatgtattgcataag       c.*240

          .         .         .         .         .         .       g.9494
 ctaaagcaacacagacactcctaggcaaagtttttgtttgtgaatagtacttgcaaaact       c.*300

          .         .         .         .         .         .       g.9554
 tgtaaattagcagatgacttttttccattgttttctccagagagaatgtgctatattttt       c.*360

          .         .         .         .         .         .       g.9614
 gtatatacaataatatttgcaactgtgaaaaacaagttgtgccatactacatggcacaga       c.*420

          .         .         .         .         .         .       g.9674
 cacaaaatattatactaatatgttgtacattcggaagaatgtgaatcaatcagtatgttt       c.*480

          .         .         .         .         .         .       g.9734
 ttagattgtattttgccttacagaaagcctttattgtaagactctgatttccctttggac       c.*540

          .         .         .         .         .         .       g.9794
 ttcatgtatattgtacagttacagtaaaattcaacctttattttctaattttttcaacat       c.*600

          .         .         .         .         .         .       g.9854
 attgtttagtgtaaagaatatttatttgaagttttattattttataaaaaagaatattta       c.*660

          .         .         .         .         .         .       g.9914
 ttttaagaggcatcttacaaattttgccccttttatgaggatgtgatagttgctgcaaat       c.*720

          .         .         .         .         .         .       g.9974
 gaggggttacagatgcatatgtccaatataaaatagaaaatatattaacgtttgaaatta       c.*780

                                                                    g.9976
 aa                                                                 c.*782

 (downstream sequence)

Legend:
Nucleotide numbering (following the rules of the HGVS for a 'Coding DNA Reference Sequence') is indicated at the right of the sequence, counting the A of the ATG translation initiating Methionine as 1. Every 10^th nucleotide is indicated by a "." above the sequence. The Sprouty homolog 2 (Drosophila) protein sequence is shown below the coding DNA sequence, with numbering indicated at the right starting with 1 for the translation initiating Methionine. Every 10^th amino acid is shown in bold. The position of introns is indicated by a vertical line, splitting the two exons. The start of the first exon (transcription initiation site) is indicated by a '\', the end of the last exon (poly-A addition site) by a '/'. The exon number is indicated above the first nucleotide(s) of the exon. To aid the description of frame shift variants, all stop codons in the +1 frame are shown in bold while all stop codons in the +2 frame are underlined.