TRK-fused gene (TFG) - coding DNA reference sequence

(used for variant description)

(last modified March 18, 2016)

This file was created to facilitate the description of sequence variants on transcript NM_001007565.2 in the TFG gene based on a coding DNA reference sequence following the HGVS recommendations.

The sequence was taken from NG_027821.1, covering TFG transcript NM_001007565.2.

Please note that introns are available by clicking on the exon numbers above the sequence.

 (upstream sequence)
                                                                    g.5005
                                                        tggtc       c.-181

 .         .         .         .         .         .                g.5065
 acgccccgcgcatgcgtgcatcggggcagctcacgcttcctaaatcccgtcatccagtga       c.-121

 .         .         .         .         .         .                g.5125
 gaaagcagggactcttcggcctaggcagccgggacccagccagccctgcgcctcgcgccg       c.-61

 .         .       | 02 .         .         .         .             g.9396
 tcgcgcatgcgtcctgg | tctttctctagagttgtatatatagaacatcctggagtccacc    c.-1

          .         .         .         .         .         .       g.9456
 ATGAACGGACAGTTGGATCTAAGTGGGAAGCTAATCATCAAAGCTCAACTTGGGGAGGAT       c.60
 M  N  G  Q  L  D  L  S  G  K  L  I  I  K  A  Q  L  G  E  D         p.20

          .         .         .         .         .         .       g.9516
 ATTCGGCGAATTCCTATTCATAATGAAGATATTACTTATGATGAATTAGTGCTAATGATG       c.120
 I  R  R  I  P  I  H  N  E  D  I  T  Y  D  E  L  V  L  M  M         p.40

          .         .         .         .         .         .       g.9576
 CAACGAGTTTTCAGAGGAAAACTTCTGAGTAATGATGAAGTAACAATAAAGTATAAAGAT       c.180
 Q  R  V  F  R  G  K  L  L  S  N  D  E  V  T  I  K  Y  K  D         p.60

      | 03   .         .         .         .         .         .    g.15741
 GAAG | ATGGAGATCTTATAACAATTTTTGATAGTTCTGACCTTTCCTTTGCAATTCAGTGC    c.240
 E  D |   G  D  L  I  T  I  F  D  S  S  D  L  S  F  A  I  Q  C      p.80

          .         .         | 04         .         .         .    g.24454
 AGTAGGATACTGAAACTGACATTATTTG | TTAATGGCCAGCCAAGACCCCTTGAATCAAGT    c.300
 S  R  I  L  K  L  T  L  F  V |   N  G  Q  P  R  P  L  E  S  S      p.100

          .         .         .         .         .         .       g.24514
 CAGGTGAAATATCTCCGTCGAGAACTGATAGAACTTCGAAATAAAGTGAATCGTTTATTG       c.360
 Q  V  K  Y  L  R  R  E  L  I  E  L  R  N  K  V  N  R  L  L         p.120

          .         .         .         .         .      | 05  .    g.28223
 GATAGCTTGGAACCACCTGGAGAACCAGGACCTTCCACCAATATTCCTGAAAATG | ATACT    c.420
 D  S  L  E  P  P  G  E  P  G  P  S  T  N  I  P  E  N  D |   T      p.140

          .         .         .         .         .         .       g.28283
 GTGGATGGTAGGGAAGAAAAGTCTGCTTCTGATTCTTCTGGAAAACAGTCTACTCAGGTT       c.480
 V  D  G  R  E  E  K  S  A  S  D  S  S  G  K  Q  S  T  Q  V         p.160

          .         .         .         .         .         .       g.28343
 ATGGCAGCAAGTATGTCTGCTTTTGATCCTTTAAAAAACCAAGATGAAATCAATAAAAAT       c.540
 M  A  A  S  M  S  A  F  D  P  L  K  N  Q  D  E  I  N  K  N         p.180

          .         .         .         . | 06       .         .    g.32306
 GTTATGTCAGCGTTTGGCTTAACAGATGATCAGGTTTCAG | GGCCACCCAGTGCTCCTGCA    c.600
 V  M  S  A  F  G  L  T  D  D  Q  V  S  G |   P  P  S  A  P  A      p.200

          .         .         .         .         .         .       g.32366
 GAAGATCGTTCAGGAACACCCGACAGCATTGCTTCCTCCTCCTCAGCAGCTCACCCACCA       c.660
 E  D  R  S  G  T  P  D  S  I  A  S  S  S  S  A  A  H  P  P         p.220

          .         .         .         .         .         .       g.32426
 GGCGTTCAGCCACAGCAGCCACCATATACAGGAGCTCAGACTCAAGCAGGTCAGATTGAA       c.720
 G  V  Q  P  Q  Q  P  P  Y  T  G  A  Q  T  Q  A  G  Q  I  E         p.240

   | 07      .         .         .         .         .         .    g.40602
 G | GTCAGATGTACCAACAGTACCAGCAACAGGCCGGCTATGGTGCACAGCAGCCGCAGGCT    c.780
 G |   Q  M  Y  Q  Q  Y  Q  Q  Q  A  G  Y  G  A  Q  Q  P  Q  A      p.260

          .         .         .         . | 08       .         .    g.43879
 CCACCTCAGCAGCCTCAACAGTATGGTATTCAGTATTCAG | CAAGCTATAGTCAGCAGACT    c.840
 P  P  Q  Q  P  Q  Q  Y  G  I  Q  Y  S  A |   S  Y  S  Q  Q  T      p.280

          .         .         .         .         .         .       g.43939
 GGACCTCAACAACCTCAGCAGTTCCAGGGATATGGCCAGCAACCAACTTCCCAGGCACCA       c.900
 G  P  Q  Q  P  Q  Q  F  Q  G  Y  G  Q  Q  P  T  S  Q  A  P         p.300

          .         .         .         .         .         .       g.43999
 GCTCCTGCCTTTTCTGGTCAGCCTCAACAACTGCCTGCTCAGCCGCCACAGCAGTACCAG       c.960
 A  P  A  F  S  G  Q  P  Q  Q  L  P  A  Q  P  P  Q  Q  Y  Q         p.320

          .         .         .         .         .         .       g.44059
 GCGAGCAATTATCCTGCACAAACTTACACTGCCCAAACTTCTCAGCCTACTAATTATACT       c.1020
 A  S  N  Y  P  A  Q  T  Y  T  A  Q  T  S  Q  P  T  N  Y  T         p.340

          .         .         .         .         .         .       g.44119
 GTGGCTCCTGCCTCTCAACCTGGAATGGCTCCAAGCCAACCTGGGGCCTATCAACCAAGA       c.1080
 V  A  P  A  S  Q  P  G  M  A  P  S  Q  P  G  A  Y  Q  P  R         p.360

          .         .         .         .         .         .       g.44179
 CCAGGTTTTACTTCACTTCCTGGAAGTACCATGACCCCTCCTCCAAGTGGGCCTAATCCT       c.1140
 P  G  F  T  S  L  P  G  S  T  M  T  P  P  P  S  G  P  N  P         p.380

          .         .         .         .         .         .       g.44239
 TATGCGCGTAACCGTCCTCCCTTTGGTCAGGGCTATACCCAACCTGGACCTGGTTATCGA       c.1200
 Y  A  R  N  R  P  P  F  G  Q  G  Y  T  Q  P  G  P  G  Y  R         p.400

                                                                    g.44242
 TAA                                                                c.1203
 X                                                                  p.400

          .         .         .         .         .         .       g.44302
 ggaggctcctctacaccaattaatgtagctgctagctattggcctcccaaaagactccag       c.*60

          .         .         .         .         .         .       g.44362
 tactattttaatttgtattgaagaagttcagaaatttaaaagcagagcattttttatgat       c.*120

          .         .         .         .         .         .       g.44422
 atcattgttggtgttaattgaaagtataatttgctggaacacaaagaccaaaatgaaagt       c.*180

          .         .         .         .         .         .       g.44482
 tttttcctccctgcttaaaaatgtagcagcttcttagttactttggaacactactcttac       c.*240

          .         .         .         .         .         .       g.44542
 atgtataaagtgattgacttgactttctagcttcccttgtccggaggatattaaaatgct       c.*300

          .         .         .         .         .         .       g.44602
 agggtgaggtttagccatcttacttggctttttactattaacatgatgtactaaagtaga       c.*360

          .         .         .         .         .         .       g.44662
 gccctttgagaatacaagatattatgtataaaatgtaacactgatgataggttaataaag       c.*420

          .                                                         g.44678
 atgattgaatccatta                                                   c.*436

 (downstream sequence)

Legend:
Nucleotide numbering (following the rules of the HGVS for a 'Coding DNA Reference Sequence') is indicated at the right of the sequence, counting the A of the ATG translation initiating Methionine as 1. Every 10^th nucleotide is indicated by a "." above the sequence. The TRK-fused gene protein sequence is shown below the coding DNA sequence, with numbering indicated at the right starting with 1 for the translation initiating Methionine. Every 10^th amino acid is shown in bold. The position of introns is indicated by a vertical line, splitting the two exons. The start of the first exon (transcription initiation site) is indicated by a '\', the end of the last exon (poly-A addition site) by a '/'. The exon number is indicated above the first nucleotide(s) of the exon. To aid the description of frame shift variants, all stop codons in the +1 frame are shown in bold while all stop codons in the +2 frame are underlined.