tumor protein p53 (TP53) - coding DNA reference sequence

(used for variant description)

(last modified August 24, 2015)

This file was created to facilitate the description of sequence variants on transcript NM_000546.5 in the TP53 gene based on a coding DNA reference sequence following the HGVS recommendations.

The sequence was taken from NG_017013.2, covering TP53 transcript NM_000546.5.

Please note that introns are available by clicking on the exon numbers above the sequence.

 (upstream sequence)
                                         .         .                g.5022
                                       gatgggattggggttttcccct       c.-181

 .         .         .         .         .         .                g.5082
 cccatgtgctcaagactggcgctaaaagttttgagcttctcaaaagtctagagccaccgt       c.-121

 .         .         .         .         .         .                g.5142
 ccagggagcaggtagctgctgggctccggggacactttgcgttcgggctgggagcgtgct       c.-61

 .         .         .         .  | 02      .         .             g.15956
 ttccacgacggtgacacgcttccctggattgg | cagccagactgccttccgggtcactgcc    c.-1

          .         .         .         .         .         .       g.16016
 ATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGCCCCCTCTGAGTCAGGAAACATTTTCA       c.60
 M  E  E  P  Q  S  D  P  S  V  E  P  P  L  S  Q  E  T  F  S         p.20

          .     | 03   .         .       | 04 .         .         . g.16302
 GACCTATGGAAACT | ACTTCCTGAAAACAACGTTCTG | TCCCCCTTGCCGTCCCAAGCAATG c.120
 D  L  W  K  L  |  L  P  E  N  N  V  L   | S  P  L  P  S  Q  A  M   p.40

          .         .         .         .         .         .       g.16362
 GATGATTTGATGCTGTCCCCGGACGATATTGAACAATGGTTCACTGAAGACCCAGGTCCA       c.180
 D  D  L  M  L  S  P  D  D  I  E  Q  W  F  T  E  D  P  G  P         p.60

          .         .         .         .         .         .       g.16422
 GATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCCGTGGCCCCTGCACCAGCAGCTCCT       c.240
 D  E  A  P  R  M  P  E  A  A  P  P  V  A  P  A  P  A  A  P         p.80

          .         .         .         .         .         .       g.16482
 ACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAG       c.300
 T  P  A  A  P  A  P  A  P  S  W  P  L  S  S  S  V  P  S  Q         p.100

          .         .         .         .         .         .       g.16542
 AAAACCTACCAGGGCAGCTACGGTTTCCGTCTGGGCTTCTTGCATTCTGGGACAGCCAAG       c.360
 K  T  Y  Q  G  S  Y  G  F  R  L  G  F  L  H  S  G  T  A  K         p.120

          .      | 05  .         .         .         .         .    g.17359
 TCTGTGACTTGCACG | TACTCCCCTGCCCTCAACAAGATGTTTTGCCAACTGGCCAAGACC    c.420
 S  V  T  C  T   | Y  S  P  A  L  N  K  M  F  C  Q  L  A  K  T      p.140

          .         .         .         .         .         .       g.17419
 TGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGCGTCCGCGCCATG       c.480
 C  P  V  Q  L  W  V  D  S  T  P  P  P  G  T  R  V  R  A  M         p.160

          .         .         .         .         .         .       g.17479
 GCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGCCCCCACCATGAG       c.540
 A  I  Y  K  Q  S  Q  H  M  T  E  V  V  R  R  C  P  H  H  E         p.180

          .          | 06        .         .         .         .    g.17620
 CGCTGCTCAGATAGCGATG | GTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGGAAAT    c.600
 R  C  S  D  S  D  G |   L  A  P  P  Q  H  L  I  R  V  E  G  N      p.200

          .         .         .         .         .         .       g.17680
 TTGCGTGTGGAGTATTTGGATGACAGAAACACTTTTCGACATAGTGTGGTGGTGCCCTAT       c.660
 L  R  V  E  Y  L  D  D  R  N  T  F  R  H  S  V  V  V  P  Y         p.220

          .   | 07     .         .         .         .         .    g.18308
 GAGCCGCCTGAG | GTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGT    c.720
 E  P  P  E   | V  G  S  D  C  T  T  I  H  Y  N  Y  M  C  N  S      p.240

          .         .         .         .         .         .       g.18368
 TCCTGCATGGGCGGCATGAACCGGAGGCCCATCCTCACCATCATCACACTGGAAGACTCC       c.780
 S  C  M  G  G  M  N  R  R  P  I  L  T  I  I  T  L  E  D  S         p.260

    | 08     .         .         .         .         .         .    g.18771
 AG | TGGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCCTGGGAGA    c.840
 S  |  G  N  L  L  G  R  N  S  F  E  V  R  V  C  A  C  P  G  R      p.280

          .         .         .         .         .         .       g.18831
 GACCGGCGCACAGAGGAAGAGAATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCC       c.900
 D  R  R  T  E  E  E  N  L  R  K  K  G  E  P  H  H  E  L  P         p.300

          .          | 09        .         .         .         .    g.18983
 CCAGGGAGCACTAAGCGAG | CACTGCCCAACAACACCAGCTCCTCTCCCCAGCCAAAGAAG    c.960
 P  G  S  T  K  R  A |   L  P  N  N  T  S  S  S  P  Q  P  K  K      p.320

          .         .         .    | 10    .         .         .    g.21862
 AAACCACTGGATGGAGAATATTTCACCCTTCAG | ATCCGTGGGCGTGAGCGCTTCGAGATG    c.1020
 K  P  L  D  G  E  Y  F  T  L  Q   | I  R  G  R  E  R  F  E  M      p.340

          .         .         .         .         .         .       g.21922
 TTCCGAGAGCTGAATGAGGCCTTGGAACTCAAGGATGCCCAGGCTGGGAAGGAGCCAGGG       c.1080
 F  R  E  L  N  E  A  L  E  L  K  D  A  Q  A  G  K  E  P  G         p.360

          .         . | 11       .         .         .         .    g.22900
 GGGAGCAGGGCTCACTCCAG | CCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCAT    c.1140
 G  S  R  A  H  S  S  |  H  L  K  S  K  K  G  Q  S  T  S  R  H      p.380

          .         .         .         .                           g.22942
 AAAAAACTCATGTTCAAGACAGAAGGGCCTGACTCAGACTGA                         c.1182
 K  K  L  M  F  K  T  E  G  P  D  S  D  X                           p.393

          .         .         .         .         .         .       g.23002
 cattctccacttcttgttccccactgacagcctcccacccccatctctccctcccctgcc       c.*60

          .         .         .         .         .         .       g.23062
 attttgggttttgggtctttgaacccttgcttgcaataggtgtgcgtcagaagcacccag       c.*120

          .         .         .         .         .         .       g.23122
 gacttccatttgctttgtcccggggctccactgaacaagttggcctgcactggtgttttg       c.*180

          .         .         .         .         .         .       g.23182
 ttgtggggaggaggatggggagtaggacataccagcttagattttaaggtttttactgtg       c.*240

          .         .         .         .         .         .       g.23242
 agggatgtttgggagatgtaagaaatgttcttgcagttaagggttagtttacaatcagcc       c.*300

          .         .         .         .         .         .       g.23302
 acattctaggtaggggcccacttcaccgtactaaccagggaagctgtccctcactgttga       c.*360

          .         .         .         .         .         .       g.23362
 attttctctaacttcaaggcccatatctgtgaaatgctggcatttgcacctacctcacag       c.*420

          .         .         .         .         .         .       g.23422
 agtgcattgtgagggttaatgaaataatgtacatctggccttgaaaccaccttttattac       c.*480

          .         .         .         .         .         .       g.23482
 atggggtctagaacttgacccccttgagggtgcttgttccctctccctgttggtcggtgg       c.*540

          .         .         .         .         .         .       g.23542
 gttggtagtttctacagttgggcagctggttaggtagagggagttgtcaagtctctgctg       c.*600

          .         .         .         .         .         .       g.23602
 gcccagccaaaccctgtctgacaacctcttggtgaaccttagtacctaaaaggaaatctc       c.*660

          .         .         .         .         .         .       g.23662
 accccatcccacaccctggaggatttcatctcttgtatatgatgatctggatccaccaag       c.*720

          .         .         .         .         .         .       g.23722
 acttgttttatgctcagggtcaatttcttttttcttttttttttttttttttctttttct       c.*780

          .         .         .         .         .         .       g.23782
 ttgagactgggtctcgctttgttgcccaggctggagtggagtggcgtgatcttggcttac       c.*840

          .         .         .         .         .         .       g.23842
 tgcagcctttgcctccccggctcgagcagtcctgcctcagcctccggagtagctgggacc       c.*900

          .         .         .         .         .         .       g.23902
 acaggttcatgccaccatggccagccaacttttgcatgttttgtagagatggggtctcac       c.*960

          .         .         .         .         .         .       g.23962
 agtgttgcccaggctggtctcaaactcctgggctcaggcgatccacctgtctcagcctcc       c.*1020

          .         .         .         .         .         .       g.24022
 cagagtgctgggattacaattgtgagccaccacgtccagctggaagggtcaacatctttt       c.*1080

          .         .         .         .         .         .       g.24082
 acattctgcaagcacatctgcattttcaccccacccttcccctccttctccctttttata       c.*1140

          .         .         .         .         .         .       g.24142
 tcccatttttatatcgatctcttattttacaataaaactttgctgccacctgtgtgtctg       c.*1200

                                                                    g.24149
 aggggtg                                                            c.*1207

 (downstream sequence)

Legend:
Nucleotide numbering (following the rules of the HGVS for a 'Coding DNA Reference Sequence') is indicated at the right of the sequence, counting the A of the ATG translation initiating Methionine as 1. Every 10^th nucleotide is indicated by a "." above the sequence. The Tumor protein p53 protein sequence is shown below the coding DNA sequence, with numbering indicated at the right starting with 1 for the translation initiating Methionine. Every 10^th amino acid is shown in bold. The position of introns is indicated by a vertical line, splitting the two exons. The start of the first exon (transcription initiation site) is indicated by a '\', the end of the last exon (poly-A addition site) by a '/'. The exon number is indicated above the first nucleotide(s) of the exon. To aid the description of frame shift variants, all stop codons in the +1 frame are shown in bold while all stop codons in the +2 frame are underlined.